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Literature summary for 2.1.1.116 extracted from

  • Inui, T.; Tamura, K.; Fujii, N.; Morishige, T.; Sato, F.
    Overexpression of Coptis japonica norcoclaurine 6-O-methyltransferase overcomes the rate-limiting step in Benzylisoquinoline alkaloid biosynthesis in cultured Eschscholzia californica (2007), Plant Cell Physiol., 48, 252-262.
    View publication on PubMed
Search for more literature for 2.1.1.116

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA of Coptis japonica 4'OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens Coptis japonica
into the pET-21d vector for expression in Escherichia coli BL21DE3 cells Coptis japonica
into the pET-41a+ vector for expression in Escherichia coli BL21DE3 cells Eschscholzia californica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + (S)-reticuline Eschscholzia californica
-
 S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
S-adenosyl-L-homocysteine + (S)-reticuline Coptis japonica
-
 S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine Coptis japonica
-
 S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?

Organism

Organism UniProt Comment Textmining
Coptis japonica Q9LEL5
-
-
Eschscholzia californica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Coptis japonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + (S)-reticuline
-
 Eschscholzia californica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
S-adenosyl-L-homocysteine + (S)-reticuline
-
 Coptis japonica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 Coptis japonica S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?

Synonyms

Synonyms Comment Organism
3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
 Eschscholzia californica
3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
 Coptis japonica
4'OMT
-
 Eschscholzia californica
4'OMT
-
 Coptis japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 OMT enzyme assay Eschscholzia californica
30
-
 OMT enzyme assay Coptis japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 OMT enzyme assay Eschscholzia californica
7.5
-
 OMT enzyme assay Coptis japonica