Literature summary for 2.1.1.113 extracted from

O'Gara, M.; Adams, G.M.; Gong, W.; Kobayashi, R.; Blumenthal, R.M.; Cheng, X.
Expression, purification, mass spectrometry, crystallization and multiwavelength anomalous diffraction of selenomethionyl PvuII DNA methyltransferase (cytosine-N4-specific) (1997), Eur. J. Biochem., 247, 1009-1018.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli DH10B	Proteus vulgaris

Crystallization (Commentary)

Crystallization (Comment)	Organism
native and selenomethionyl M.PvuII crystallized separately as binary complexs of the methyl donor S-adenosyl-L-methionine in the monoclinic space group P2. Hanging-drop vapor-diffusion technique	Proteus vulgaris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36847	-	x * 36847, MALDI-TOF mass spectrometry	Proteus vulgaris

Organism

Organism	UniProt	Comment	Textmining
Proteus vulgaris	-	enzyme M.PvuII	-

Purification (Commentary)

Purification (Comment)	Organism
native and selenomethionyl M.PvuII	Proteus vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + [DNA]-cytosine	-	Proteus vulgaris	S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine	-	?

Subunits

Subunits	Comment	Organism
?	x * 36847, MALDI-TOF mass spectrometry	Proteus vulgaris

Synonyms

Synonyms	Comment	Organism
M.PvuII	-	Proteus vulgaris

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Release 2023.1 (January 2023)