Literature summary for 2.1.1.111 extracted from

Rohde, B.; Hans, J.; Martens, S.; Baumert, A.; Hunziker, P.; Matern, U.
Anthranilate N-methyltransferase, a branch-point enzyme of acridone biosynthesis (2008), Plant J., 53, 541-553.

Search for more literature for 2.1.1.111

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli, BL21 cells for induction of recombinant protein expression	Ruta graveolens

Protein Variants

Protein Variants	Comment	Organism
N298E	generated by site-directed mutagenesis	Ruta graveolens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	S-adenosyl-L-methionine	wild-type	Ruta graveolens
0.003	-	S-adenosyl-L-methionine	N298E mutant	Ruta graveolens
0.007	-	anthranilate	wild-type	Ruta graveolens
0.024	-	anthranilate	N298E mutant	Ruta graveolens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	73% relative enzyme activity in presence of 1.5 mM, significantly reduced	Ruta graveolens
Co2+	activity reduced in presence of 1.5 mM, 25% relative activity retained	Ruta graveolens
Cu2+	activity abolished in presence of 1.5 mM of	Ruta graveolens
Fe2+	activity nearly abolished in presence of 1.5 mM of	Ruta graveolens
Fe3+	activity abolished in presence of 1.5 mM of	Ruta graveolens
Mg2+	activity independent in presence of 1.5 mM of, 90% relative activity retained	Ruta graveolens
Zn2+	activity abolished in presence of 1.5 mM of	Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration, SDS-PAGE	Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + anthranilate	Ruta graveolens	first specific step in acridone alkaloid biosynthesis	S-adenosyl-L-homocysteine + N-methylanthranilate	-	?

Organism

Organism	UniProt	Comment	Textmining
Ruta graveolens	A9X7L0	line R-20	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, SDS-PAGE, wild-type and mutant protein	Ruta graveolens

Source Tissue

Source Tissue	Comment	Organism	Textmining
carpel	lining the inside of	Ruta graveolens	-
cell suspension culture	crude extracts of total plants, elicited, 2 years old	Ruta graveolens	-
flower	high mRNA expression and enzyme activity	Ruta graveolens	-
leaf	moderate mRNA expression	Ruta graveolens	-
ovary	central vascular bundles of, around cup-like structures in the outer ovary wall representing secretory oil glands	Ruta graveolens	-
root	rhizodermis of older root parts, endodermis of younger root parts, high mRNA expression and enzyme activity	Ruta graveolens	-
stem	vascular region of secondary and primary shoots, moderate mRNA expression, high enzyme activity	Ruta graveolens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	in-gel digestion and peptide sequencing by mass spectrometry, Western Blot and phylogenetic analysis, assays conducted at 32°C in 73.5 mM glycine-NaOH buffer at pH 8.9, HPLC separation of assay extracts, list of compounds that are not accepted as substrate in assay	Ruta graveolens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + anthranilate	first specific step in acridone alkaloid biosynthesis	Ruta graveolens	S-adenosyl-L-homocysteine + N-methylanthranilate	-	?
S-adenosyl-L-methionine + anthranilate	first specific step in alkaloid biosynthesis, substrate specificity determines the course of acridone biosynthesis	Ruta graveolens	S-adenosyl-L-homocysteine + N-methylanthranilate	-	?

Synonyms

Synonyms	Comment	Organism
ANMT	-	Ruta graveolens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
32	-	assay at	Ruta graveolens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	activity optimum at	Ruta graveolens

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Release 2023.1 (January 2023)