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Literature summary for 2.1.1.107 extracted from

  • Storbeck, S.; Saha, S.; Krausze, J.; Klink, B.U.; Heinz, D.W.; Layer, G.
    Crystal structure of the heme d1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferases (2011), J. Biol. Chem., 286, 26754-26767.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using Tris, pH 8.0, 24% (w/v) PEG 6000 for enzyme without substrate, and 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 1.26 M ammonium sulfate for enzyme in complex with uroporphyrinogen III Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E114Q the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
G189K the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa
G189N the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa
H161F the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
K102A the mutant shows no activity and strongly reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa
M186L the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa
R111K the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa
R149K the mutant shows no activity compared to the wild type enzyme Pseudomonas aeruginosa
R51K the mutant shows reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
 Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P95417
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Pseudomonas aeruginosa 2 S-adenosyl-L-homocysteine + precorrin-2
-
 ir

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
NirE
-
 Pseudomonas aeruginosa
S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase
-
 Pseudomonas aeruginosa