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Literature summary for 2.1.1.103 extracted from

  • Saen-Oon, S.; Lee, S.G.; Jez, J.M.; Guallar, V.
    An alternative mechanism for the methylation of phosphoethanolamine catalyzed by Plasmodium falciparum phosphoethanolamine methyltransferase (2014), J. Biol. Chem., 289, 33815-33825.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.103

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
D128A mutant in complex with S-adenosylhomocysteine and either phosphoethanolamine or phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 8000, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl)phosphine, and 5 mM S-adenosyl-L-homocysteine Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
D128A compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 91% activity) Plasmodium falciparum
D128E compared to the wild type enzyme, the mutant shows about 90% loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 182% activity) Plasmodium falciparum
D128N compared to the wild type enzyme, the mutant shows complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 24% activity) Plasmodium falciparum
D128Q compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 89% activity) Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
0.181
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
0.773
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
0.786
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
7.99
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
21.17
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + ethanolamine phosphate Plasmodium falciparum
-
 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q6T755
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + ethanolamine phosphate
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
S-adenosyl-L-methionine + N,N-dimethylethanolamine phosphate
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + phosphocholine
-
 ?

Synonyms

Synonyms Comment Organism
phosphoethanolamine methyltransferase
-
 Plasmodium falciparum
PMT
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
0.022
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
0.367
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
1.483
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
1.82
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
3.08
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum

General Information

General Information Comment Organism
physiological function the enzyme is essential for normal growth and development Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00025
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
0.00271
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
0.474
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
1.887
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
17.04
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
33.64
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum