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Literature summary for 2.1.1.103 extracted from

  • Lee, S.G.; Haakenson, W.; McCarter, J.P.; Williams, D.J.; Hresko, M.C.; Jez, J.M.
    Thermodynamic evaluation of ligand binding in the plant-like phosphoethanolamine methyltransferases of the parasitic nematode Haemonchus contortus (2011), J. Biol. Chem., 286, 38060-38068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.103

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta II(DE3) pLysS cells Haemonchus contortus

Inhibitors

Inhibitors Comment Organism Structure
amodiaquine i.e. 4-[(7-chloroquinolin-4-yl)amino]-2-[(diethylamino)methyl]phenol Haemonchus contortus
diphenhydramine i.e. 2-(diphenylmethoxy)-N,N-dimethylethanamine Haemonchus contortus
miltefosine i.e. hexadecyl 2-(trimethylammonio)ethyl phosphate Haemonchus contortus
additional information isozyme PMT1 is not inhibited by diphenhydramine Haemonchus contortus
sinefungin
-
 Haemonchus contortus
tacrine i.e. 1,2,3,4-tetrahydroacridin-9-amine Haemonchus contortus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.106
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
0.217
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
0.256
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
0.277
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
0.424
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 1 * 48000, isozyme PMT2, SDS-PAGE Haemonchus contortus
49400
-
 1 * 49400, isozyme PMT2, calculated from amino acid sequence Haemonchus contortus
51900
-
 isozyme PMT2, gel filtration Haemonchus contortus
52300
-
 isozyme PMT1, gel filtration Haemonchus contortus
53100
-
 1 * 53100, isozyme PMT1, calculated from amino acid sequence Haemonchus contortus
55000
-
 1 * 55000, isozyme PMT1, SDS-PAGE Haemonchus contortus

Organism

Organism UniProt Comment Textmining
Haemonchus contortus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration Haemonchus contortus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + phosphoethanolamine overall reaction Haemonchus contortus 3 S-adenosyl-L-homocysteine + phosphocholine
-
 ?
additional information isozyme PMT2 does not accept phosphoethanolamine as substrate Haemonchus contortus ?
-
 ?
S-adenosyl-L-methionine + phosphodimethylethanolamine reaction catalyzed by isozyme PMT2 Haemonchus contortus S-adenosyl-L-homocysteine + phosphocholine
-
 ?
S-adenosyl-L-methionine + phosphoethanolamine reaction catalyzed by isozyme PMT1 Haemonchus contortus S-adenosyl-L-homocysteine + phosphomonomethylethanolamine
-
 ?
S-adenosyl-L-methionine + phosphomonomethylethanolamine reaction catalyzed by isozyme PMT2 Haemonchus contortus S-adenosyl-L-homocysteine + phosphodimethylethanolamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48000, isozyme PMT2, SDS-PAGE Haemonchus contortus
monomer 1 * 49400, isozyme PMT2, calculated from amino acid sequence Haemonchus contortus
monomer 1 * 53100, isozyme PMT1, calculated from amino acid sequence Haemonchus contortus
monomer 1 * 55000, isozyme PMT1, SDS-PAGE Haemonchus contortus

Synonyms

Synonyms Comment Organism
phosphoethanolamine methyltransferase
-
 Haemonchus contortus
PMT1 isozyme PMT1 contains a methyltransferase domain in the N-terminal half of the protein Haemonchus contortus
PMT2 isozyme PMT2 encodes a C-terminal methyltransferase domain Haemonchus contortus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.28
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
4.13
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
4.9
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
5.82
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
8.63
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus

pI Value

Organism Comment pI Value Maximum pI Value
Haemonchus contortus isozyme PMT1, calculated from amino acid sequence
-
 5.3
Haemonchus contortus isozyme PMT2, calculated from amino acid sequence
-
 5.5

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0125
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus miltefosine
0.013
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus diphenhydramine
0.0338
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus sinefungin
0.0915
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus sinefungin
0.1252
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus miltefosine
3.1
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus amodiaquine
3.4
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus amodiaquine
3.5
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus tacrine
23.4
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus tacrine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.24
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
11.56
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
19.05
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
22.72
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
81.45
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus