Cloned (Comment) | Organism |
---|---|
in Escherichia coli | Triticum aestivum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | several point mutations and N- and C-terminal domains investigated, N-terminal domain catalyzes all three activities, regulatory role for C-terminal domain suggested | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
S-adenosyl-L-methionine | - |
Triticum aestivum | |
0.065 | - |
Ethanolamine phosphate | - |
Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
calculated from DNA sequence | Triticum aestivum |
65000 | - |
SDS-PAGE, enzyme from E. coli, contains of 57 kD enzyme protein + 8 kD His-tag | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + ethanolamine phosphate | Triticum aestivum | involved in synthesis of phosphocholine and phosphatidylcholine | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | Q8VYX1 | - |
- |
Purification (Comment) | Organism |
---|---|
from E. coli, His-tag used for affinity chromatography | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate | - |
Triticum aestivum | S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate | - |
? | |
S-adenosyl-L-methionine + ethanolamine phosphate | - |
Triticum aestivum | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + ethanolamine phosphate | involved in synthesis of phosphocholine and phosphatidylcholine | Triticum aestivum | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
S-adenosyl-L-methionine + phosphodimethylethanolamine | - |
Triticum aestivum | S-adenosyl-L-homocysteine + phosphocholine | - |
? |