Application | Comment | Organism |
---|---|---|
pharmacology | tylosin fermentation, antibiotic biosynthesis | Streptomyces fradiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
20-Dihydro-23-deoxymycaminosyltylonolide | - |
Streptomyces fradiae | |
20-dihydrolactenocin | inhibition of macrocin formation | Streptomyces fradiae | |
20-Dihydromacrocin | - |
Streptomyces fradiae | |
23-demycinosyloxytylosin | - |
Streptomyces fradiae | |
23-Deoxy-O-mycaminosyltylonolide | - |
Streptomyces fradiae | |
A9145C | - |
Streptomyces fradiae | |
demycinosyltylosin | - |
Streptomyces fradiae | |
Fe2+ | - |
Streptomyces fradiae | |
lactenocin | inhibition of macrocin formation | Streptomyces fradiae | |
macrocin | inhibition of lactenocin formation | Streptomyces fradiae | |
mycaminosyltylactone | - |
Streptomyces fradiae | |
mycaminosyltylonolide | - |
Streptomyces fradiae | |
O-Mycaminosyltylonolide | - |
Streptomyces fradiae | |
S-adenosyl-L-homocysteine | not D-enantiomer | Streptomyces fradiae | |
sinefungin | competitive inhibition, poor inhibitor | Streptomyces fradiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
Demethylmacrocin | - |
Streptomyces fradiae | |
0.01 | - |
demethyllactenocin | - |
Streptomyces fradiae | |
0.111 | - |
S-adenosylmethionine | - |
Streptomyces fradiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximal activity | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
3 * 42000, SDS-PAGE | Streptomyces fradiae |
122000 | 126000 | gel filtration | Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + demethylmacrocin | Streptomyces fradiae | enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose | S-adenosyl-L-homocysteine + macrocin | - |
? | |
S-adenosyl-L-methionine + demethylmacrocin | Streptomyces fradiae | penultimate step in tylosin biosynthesis | S-adenosyl-L-homocysteine + macrocin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
- |
Streptomyces fradiae |
Storage Stability | Organism |
---|---|
4°C, quite stable in presence of S-adenosylmethionine and 10% ethanol, pH 7.0-8.0, stable for a minimum of 2 months | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + demethyllactenocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + lactenocin | - |
? | |
S-adenosyl-L-methionine + demethylmacrocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? | |
S-adenosyl-L-methionine + demethylmacrocin | enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose | Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? | |
S-adenosyl-L-methionine + demethylmacrocin | penultimate step in tylosin biosynthesis | Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 42000, SDS-PAGE | Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Streptomyces fradiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
lactenocin | fixed substrate demethylmacrocin | Streptomyces fradiae | |
0.024 | - |
lactenocin | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
0.037 | - |
macrocin | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
0.057 | - |
A9145C | fixed substrate demethylmacrocin | Streptomyces fradiae | |
0.065 | - |
mycaminosyltylonolide | - |
Streptomyces fradiae | |
0.067 | - |
macrocin | fixed substrate demethyllactenocin | Streptomyces fradiae | |
0.073 | - |
23-Deoxy-O-mycaminosyltylonolide | - |
Streptomyces fradiae | |
0.077 | - |
sinefungin | - |
Streptomyces fradiae | |
0.108 | - |
A9145C | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
0.11 | - |
S-adenosyl-L-homocysteine | fixed substrate demethylmacrocin | Streptomyces fradiae | |
0.186 | - |
23-demycinosyloxytylosin | - |
Streptomyces fradiae | |
0.226 | - |
S-adenosyl-L-homocysteine | fixed substrate S-adenosylmethionine | Streptomyces fradiae |