Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.100 extracted from

  • Buckner, F.S.; Kateete, D.P.; Lubega, G.W.; Van Voorhis, W.C.; Yokoyama, K.
    Trypanosoma brucei prenylated-protein carboxyl methyltransferase prefers farnesylated substrates (2002), Biochem. J., 367, 809-816.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.100

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
n-octyl beta-glucoside 0.2%, 55-75% inhibition Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine recombinant enzyme Trypanosoma brucei
0.0014
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine native enzyme Trypanosoma brucei
0.0023
-
 N-acetyl-S-geranylgeranyl-L-cysteine recombinant enzyme Trypanosoma brucei
0.004
-
 N-acetyl-S-geranylgeranyl-L-cysteine native enzyme Trypanosoma brucei
0.0115
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine native enzyme Mus musculus
0.0116
-
 N-acetyl-S-geranylgeranyl-L-cysteine native enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28301
-
 x * 28301, deduced from nucleotide sequence Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Trypanosoma brucei
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-acetyl-S-geranylgeranyl-L-cysteine
-
 Mus musculus S-adenosyl-L-homocysteine + N-acetyl-S-geranylgeranyl-L-cysteine methyl ester
-
 ?
S-adenosyl-L-methionine + N-acetyl-S-geranylgeranyl-L-cysteine
-
 Trypanosoma brucei S-adenosyl-L-homocysteine + N-acetyl-S-geranylgeranyl-L-cysteine methyl ester
-
 ?
S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine
-
 Mus musculus S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester
-
 ?
S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine
-
 Trypanosoma brucei S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester
-
 ?
S-adenosylmethionine + N-acetyl-S-(E,E)-farnesyl-L-cysteine
-
 Mus musculus S-adenosylhomocysteine + N-acetyl-S-(E,E)-farnesyl-L-cysteine methyl ester
-
 ?
S-adenosylmethionine + N-acetyl-S-(E,E)-farnesyl-L-cysteine recombinant enzyme: 50fold higher activity than with N-acetyl-S-geranylgeranyl-L-cysteine, native enzyme: 40fold higher activity than with N-acetyl-S-geranylgeranyl-L-cysteine Trypanosoma brucei S-adenosylhomocysteine + N-acetyl-S-(E,E)-farnesyl-L-cysteine methyl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 28301, deduced from nucleotide sequence Trypanosoma brucei