Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
His-tagged K100A/E101A/E103A mutant, sitting drop vapor diffusion method, PEG3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D197A | active site residue | Homo sapiens |
K100A/E101A/E103A | His-tagged version, crystallization facilitated by reducing the entropy of three surface exposed residues | Homo sapiens |
S201A | active site residue | Homo sapiens |
S213A | active site residue | Homo sapiens |
Y20A | active site residue | Homo sapiens |
Y20F | active site residue | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
S-adenosyl-L-methionine | 0.0018-0.0022 mM, wild type protein, pH 8.6, 37°C | Homo sapiens | |
0.005 | - |
S-adenosyl-L-methionine | K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.028 | - |
S-adenosyl-L-methionine | Y20F mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.105 | - |
nicotinamide | K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.24 | - |
nicotinamide | Y20F mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.4 | - |
nicotinamide | 0.43-0.38 mM, wild type protein, pH 8.6, 37°C | Homo sapiens | |
0.63 | - |
nicotinamide | Y20A mutant protein, pH 8.6, 37°C | Homo sapiens | |
2.73 | - |
S-adenosyl-L-methionine | D197A mutant protein, pH 8.6, 37°C | Homo sapiens | |
3.5 | - |
S-adenosyl-L-methionine | Y20A mutant protein, pH 8.6, 37°C | Homo sapiens | |
11.8 | - |
nicotinamide | D197A mutant protein, pH 8.6, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P40261 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged K100A/E101A/E103A mutant, immobilized metal ion affinity chromatography (Ni2+), gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0011 | - |
Y20A mutant protein, pH 8.6, 37°C | Homo sapiens |
0.0042 | - |
D197A mutant protein, pH 8.6, 37°C | Homo sapiens |
0.077 | - |
Y20F mutant protein, pH 8.6, 37°C | Homo sapiens |
0.14 | - |
S201A mutant protein, pH 8.6, 37°C | Homo sapiens |
0.15 | - |
wild type protein, pH 8.6, 37°C | Homo sapiens |
0.18 | - |
K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens |
0.21 | - |
S213A mutant protein, pH 8.6, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + S-adenosyl-L-methionine | - |
Homo sapiens | 1-methylnicotinamide + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide N-methyltransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
nicotinamide | Y20A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.036 | - |
nicotinamide | D197A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.041 | - |
nicotinamide | Y20F mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.077 | - |
nicotinamide | 0.43-0.38 mM, wild type protein, pH 8.6, 37°C | Homo sapiens | |
0.083 | - |
nicotinamide | K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
nicotinamide | D197A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.005 | - |
nicotinamide | Y20A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.009 | - |
S-adenosyl-L-methionine | Y20A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.013 | - |
S-adenosyl-L-methionine | D197A mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.17 | - |
nicotinamide | Y20F mutant protein, pH 8.6, 37°C | Homo sapiens | |
0.19 | - |
nicotinamide | 0.18-0.2 1/sec*mM, wild type protein, pH 8.6, 37°C | Homo sapiens | |
0.8 | - |
nicotinamide | K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens | |
1.5 | - |
S-adenosyl-L-methionine | Y20F mutant protein, pH 8.6, 37°C | Homo sapiens | |
17 | - |
S-adenosyl-L-methionine | K100A/E101A/E103A mutant protein, pH 8.6, 37°C | Homo sapiens | |
39 | - |
S-adenosyl-L-methionine | 35-43 1/sec*mM, wild type protein, pH 8.6, 37°C | Homo sapiens |