Cloned (Comment) | Organism |
---|---|
gene pflA, coexpression of pyruvate formate lyase (PFL, gene pflB, UniProt ID P09373 ) with the pyruvate formate lyase activating enzyme, as well as with appropriate electron donors flavodoxin and ferredoxin (encoded by genes fldA and fdx, respectively), in a non-ethanol-producing Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc- MTH1-DELTAT ura3-52), plasmid maps, overview. Reduced flavodoxin is the preferred electron donor for PFL, but coexpression of either of the electron donors has a positive effect on growth under aerobic conditions. Subcloning in Escherichia coli strain DH5alpha. The PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant coexpression of the enzyme with pyruvate format lyase and flavodoxin or ferredoxin in Saccharomyces cerevisiae leads to over 20fold increased expression of endogenous formate dehydrogenases FDH1 and FDH2 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine | Escherichia coli | - |
5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9N4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PFL-AE utilizes S-adenosylmethionine (SAM) and reduced flavodoxin as the cosubstrates to generate a 5'-deoxyadenosyl radical, which then activates PFL by abstracting a hydrogen atom from residue G734 | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine | - |
Escherichia coli | 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL-AE | - |
Escherichia coli |
PflA | - |
Escherichia coli |
pyruvate formate lyase activating enzyme | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | can partly substitute for flavodoxin | Escherichia coli | |
flavodoxin | preferred cofactor | Escherichia coli | |
additional information | reduced flavodoxin serves as an electron donor and SAM as a cosubstrate for PFL-AE to generate a 5'-deoxyadenosyl radical, which is responsible for PFL activation | Escherichia coli | |
S-adenosyl-L-methionine | - |
Escherichia coli |