Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | molybdopterin-containing enzyme | Pelobacter acidigallici |
Organism | UniProt | Comment | Textmining |
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Pelobacter acidigallici | - |
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Reaction | Comment | Organism | Reaction ID |
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1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene = 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene | transfer of the hydroxy group occurs via 2,4,3,4,5-hexahydroxydiphenyl ether as an intermediate. The 1-OH group of pyrogallol coordinates to the MoVI atom of Moco and is oxidized to the orthoquinone form, while H144 accepts the phenolic proton. the 2-OH group of 1,2,3,5-tetrahydroxybenzene adds to the quinone in a Michael-type reaction, which is assisted by proton transfer from D174. The substrate-cocatalyst adduct is tautomerized with the assistance of Y404 as a proton donor. In the last stage, the paraquinone form of the tetrahydroxybenzene is reduced by Mo(IV) | Pelobacter acidigallici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene | 2,4,6,3,4,5-hexahydroxydiphenyl ether and 3,4,5,3,4,5-hexahydroxydiphenyl ether can substitute for the cocatalyst 1,2,3,5-tetrahydroxybenzene in vitro. This indicates that the diphenyl ethers can intrude into the active site and initiate the catalytic cycle | Pelobacter acidigallici | 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene | - |
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