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Literature summary for 1.9.6.1 extracted from

  • Zeamari, K.; Gerbaud, G.; Grosse, S.; Fourmond, V.; Chaspoul, F.; Biaso, F.; Arnoux, P.; Sabaty, M.; Pignol, D.; Guigliarelli, B.; Burlat, B.
    Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase (2019), Biochim. Biophys. Acta Bioenerg., 1860, 402-413 .
    View publication on PubMed
Search for more literature for 1.9.6.1

Protein Variants

Protein Variants Comment Organism
K56H 11% of wild-type activity at pH 7 Cereibacter sphaeroides f. sp. denitrificans
K56M 5% of wild-type activity at pH 7 Cereibacter sphaeroides f. sp. denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 nitrate mutant K56M, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
0.05
-
 nitrate wild-type, 25°C, pH 5 Cereibacter sphaeroides f. sp. denitrificans
0.075
-
 nitrate mutant K56M, 25°C, pH 7 Cereibacter sphaeroides f. sp. denitrificans
0.11
-
 nitrate mutant K56H, 25°C, pH 5 Cereibacter sphaeroides f. sp. denitrificans
0.14
-
 nitrate mutant K56H, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
0.17
-
 nitrate wild-type, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
0.25
-
 nitrate wild-type, 25°C, pH 7 Cereibacter sphaeroides f. sp. denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Cereibacter sphaeroides f. sp. denitrificans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron relative metal content is around 1 Mo atom to 6 Fe atoms Cereibacter sphaeroides f. sp. denitrificans
Molybdenum the active site is a Mo bis-(pyranopterin guanine dinucleotide), relative metal content is around 1 Mo atom to 6 Fe atoms Cereibacter sphaeroides f. sp. denitrificans

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides f. sp. denitrificans O88111 and O88160 O88111 i.e. catalytic subunit NapA, O88160 i.e. electron transfer subunit NapB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced benzyl viologen
-
 Cereibacter sphaeroides f. sp. denitrificans nitrite + oxidized benzyl viologen + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NapAB
-
 Cereibacter sphaeroides f. sp. denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 nitrate mutant K56M, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
22
-
 nitrate mutant K56H, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
61
-
 nitrate wild-type, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans

Cofactor

Cofactor Comment Organism Structure
heme a [4Fe-4S] cluster and two c-type hemes form an intramolecular electron transfer chain that deliver electrons to the active site. Residue lysine 56 connects, through hydrogen-bonds, the [4Fe-4S] center to one of the pyranopterin ligands of the Mo-cofactor Cereibacter sphaeroides f. sp. denitrificans
[4Fe-4S]-center a [4Fe-4S] cluster and two c-type hemes form an intramolecular electron transfer chain that deliver electrons to the active site. Residue lysine 56 connects, through hydrogen-bonds, the [4Fe-4S] center to one of the pyranopterin ligands of the Mo-cofactor Cereibacter sphaeroides f. sp. denitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
155
-
 nitrate mutant K56H, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
250
-
 nitrate mutant K56M, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans
360
-
 nitrate wild-type, 25°C, pH 6 Cereibacter sphaeroides f. sp. denitrificans