Literature summary for 1.9.6.1 extracted from

Coelho, C.; Gonzalez, P.J.; Trincao, J.; Carvalho, A.L.; Najmudin, S.; Hettman, T.; Dieckman, S.; Moura, J.J.; Moura, I.; Romao, M.J.
Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis (2007), Acta Crystallogr. Sect. F, 63, 516-519.

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapour-diffusion method, crystals of the oxidized form of this enzyme are obtained using polyethylene glycol 3350 as precipitant. A single crystal diffracted to beyond 1.5 A at the ESRF (ID14-1), which is the highest resolution reported to date for a nitrate reductase. The unit-cell parameters are a = 142.2, b = 82.4, c = 96.8 A, beta = 100.7°, space group C2, and one heterodimer is present per asymmetric unit	Cupriavidus necator H16

Crystallization (Comment)

Organism

sitting-drop vapour-diffusion method, crystals of the oxidized form of this enzyme are obtained using polyethylene glycol 3350 as precipitant. A single crystal diffracted to beyond 1.5 A at the ESRF (ID14-1), which is the highest resolution reported to date for a nitrate reductase. The unit-cell parameters are a = 142.2, b = 82.4, c = 96.8 A, beta = 100.7°, space group C2, and one heterodimer is present per asymmetric unit

Cupriavidus necator H16

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Cupriavidus necator H16	-	-

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator H16	-	the genes for the periplasmic nitrate reductase are not part of the bacterial chromosome, but are located on the megaplasmid pHG1 present in the wild-type strain H16	-

Purification (Commentary)

Purification (Comment)	Organism
-	Cupriavidus necator H16

Synonyms

Synonyms	Comment	Organism
NapAB	-	Cupriavidus necator H16

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Release 2023.1 (January 2023)