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Literature summary for 1.8.99.2 extracted from

  • Kopriva, S.; Buchert, T.; Fritz, G.; Suter, M.; Weber, M.; Benda, R.; Schaller, J.; Feller, U.; Schurmann, P.; Schunemann, V.; Trautwein, A.X.; Kroneck, P.M.; Brunold, C.
    Plant adenosine 5'-phosphosulfate reductase is a novel iron-sulfur protein (2001), J. Biol. Chem., 276, 42881-42886.
    View publication on PubMed
Search for more literature for 1.8.99.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Arabidopsis thaliana
overexpression in Escherichia coli Lemna minor

Protein Variants

Protein Variants Comment Organism
C129S maximal velocity dropps to 50% of that of the wild-type enzyme Lemna minor
C248S mutation completely abolishes enzyme activity Lemna minor

General Stability

General Stability Organism
enzyme is rapidly denatured by even micromolar concentrations of ferricyanide Lemna minor

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 4 paramagnetic iron sites, which form a diamagnetic [4Fe-4S]2+ cluster Lemna minor

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Lemna minor
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30 40
-
 Lemna minor

Storage Stability

Storage Stability Organism
4°C, continous loss of activity Lemna minor
4°C, continous loss of activity, precipitation after 2-3 days Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl sulfate + dithioerythritol
-
 Lemna minor AMP + sulfite + ?
-
 ?

Synonyms

Synonyms Comment Organism
ATAPR2
-
 Arabidopsis thaliana
LMAPR
-
 Lemna minor

Cofactor

Cofactor Comment Organism Structure
flavin flavin is absent Arabidopsis thaliana
flavin flavin is absent Lemna minor