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Literature summary for 1.8.99.2 extracted from
- Immunological cross-reactivities of adenosine-5'-phosphate reductases from sulfate-reducing and sulfide-oxidizing bacteria (1991), Appl. Environ. Microbiol., 57, 727-733.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
AMP | - |
Desulfovibrio desulfuricans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Desulfovibrio vulgaris | |
| Iron | - |
Megalodesulfovibrio gigas | |
| Iron | - |
Desulfovibrio multispirans | |
| Iron | enzyme contains 6.3 mol iron per mol of enzyme | Desulfovibrio desulfuricans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 70000 + x * 26000, SDS-PAGE | Desulfovibrio desulfuricans |
| 26000 | - |
x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
| 70000 | - |
x * 70000 + x * 26000, SDS-PAGE | Desulfovibrio desulfuricans |
| 70000 | - |
x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
| 190000 | - |
gel filtration | Desulfovibrio desulfuricans |
| 220000 | - |
- |
Desulfovibrio vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus thiooxidans | - |
- |
- |
| Allochromatium vinosum | - |
- |
- |
| Desulfobulbus propionicus | - |
- |
- |
| Desulforamulus ruminis | - |
- |
- |
| Desulfosarcina variabilis | - |
- |
- |
| Desulfosporosinus orientis | - |
- |
- |
| Desulfotomaculum nigrificans | - |
- |
- |
| Desulfovibrio desulfuricans | - |
weak activity | - |
| Desulfovibrio desulfuricans | - |
G100A, ATCC27774, API, ATCC 13541, Norway | - |
| Desulfovibrio multispirans | - |
- |
- |
| Desulfovibrio vulgaris | - |
- |
- |
| Maridesulfovibrio salexigens | - |
- |
- |
| Megalodesulfovibrio gigas | - |
- |
- |
| Streptomyces lividans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| G100A | Desulfovibrio desulfuricans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Desulfovibrio desulfuricans |
| additional information | - |
- |
Allochromatium vinosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + sulfite + ferricyanide | - |
Desulfovibrio desulfuricans | adenylyl sulfate + ferrocyanide | - |
? | |
| AMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | adenylyl sulfate + ferrocyanide | - |
? | |
| AMP + sulfite + ferricyanide | - |
Megalodesulfovibrio gigas | adenylyl sulfate + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Desulfovibrio vulgaris |
| ? | x * 70000 + x * 26000, SDS-PAGE | Desulfovibrio desulfuricans |
| ? | x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Desulfovibrio desulfuricans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Desulfovibrio vulgaris | |
| FAD | - |
Megalodesulfovibrio gigas | |
| FAD | - |
Maridesulfovibrio salexigens | |
| FAD | enzyme contains 0.74 mol of FAD per mol of enzyme | Desulfovibrio desulfuricans | |
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