Literature summary for 1.8.7.2 extracted from

Gaymard, E.; Franchini, L.; Manieri, W.; Stutz, E.; Schurmann, P.
A dicistronic construct for the expression of functional spinach chloroplast ferredoxin:thioredoxin reductase in Escherichia coli (2000), Plant Sci., 158, 107-113.

Search for more literature for 1.8.7.2

Application

Application	Comment	Organism
synthesis	dicistronic construct for the heterologous expression in Escherichia coli. The coding sequences for the two mature subunits have been inserted in tandem into the expression vector. The dicistronic construct is correctly translated yielding soluble, perfectly functional FTR. The recombinant enzyme is composed of both subunits, contains the correctly inserted FeS cluster and is indistinguishable from the enzyme isolated from leaves in its capacity to activate chloroplast fructose-1,6-bisphosphatase	Spinacia oleracea

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Spinacia oleracea

Protein Variants

Protein Variants	Comment	Organism
additional information	dicistronic construct for the heterologous expression in Escherichia coli. The coding sequences for the two mature subunits have been inserted in tandem into the expression vector. The dicistronic construct is correctly translated yielding soluble, perfectly functional FTR. The recombinant enzyme is composed of both subunits, contains the correctly inserted FeS cluster and is indistinguishable from the enzyme isolated from leaves in its capacity to activate chloroplast fructose-1,6-bisphosphatase	Spinacia oleracea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	gel filtration, recombinant protein complex	Spinacia oleracea

Organism

Organism	UniProt	Comment	Textmining
Spinacia oleracea	-	-	-

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Release 2023.1 (January 2023)