Literature summary for 1.8.7.2 extracted from

Manieri, W.; Franchini, L.; Raeber, L.; Dai, S.; Stritt-Etter, A.L.; Schurmann, P.
N-terminal truncation of the variable subunit stabilizes spinach ferredoxin:thioredoxin reductase (2003), FEBS Lett., 549, 167-170.

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Protein Variants

Protein Variants	Comment	Organism
C27S	mutation in truncated, stabilized FTR mutant lacking 24 N-terminal amino acids. Mutant C27S is perfectly capable of activating FBPase and shows a thioredoxin f-concentration dependency comparable to the FTR truncation mutant	Spinacia oleracea
C84S	mutation in truncated, stabilized FTR mutant lacking 24 N-terminal amino acids. Mutant C84S is produced at about the same level as the WT protein, it is extremely labile and disintegrates very rapidly during the first steps of purifiation	Spinacia oleracea
additional information	construction two N-terminal truncation mutants by removing 16 or 24 residues from the variable subunit. The mutant proteins are readily expressed and show half-saturation values for ferredoxin and thioredoxin f comparable to wild-type. Truncation increases significantly their stability	Spinacia oleracea

Organism

Organism	UniProt	Comment	Textmining
Spinacia oleracea	P38365	variable subunit	-

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Release 2023.1 (January 2023)