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Literature summary for 1.8.7.1 extracted from
- A novel variant of ferredoxin-dependent sulfite reductase having preferred substrate specificity for nitrite in the unicellular red alga Cyanidioschyzon merolae (2009), Biochem. J., 423, 91-98.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pTrc99A for expression in Escherichia coli cells | Cyanidioschyzon merolae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0087 | - |
sulfite | - |
Cyanidioschyzon merolae |  |
| 0.221 | - |
nitrite | - |
Cyanidioschyzon merolae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Cyanidioschyzon merolae | 9507 | - |
| plastid | - |
Cyanidioschyzon merolae | 9536 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydrogen sulfide + oxidized ferredoxin + H2O | Cyanidioschyzon merolae | - |
sulfite + reduced ferredoxin + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyanidioschyzon merolae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cell extract is treated with DEAE-cellulose in a batchwise manner, the unbound fraction is fractionated by ammonium sulfate precipitation, anion-exchange and hydrophobic column chromatography are applied | Cyanidioschyzon merolae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Cyanidioschyzon merolae | - |
| root | - |
Cyanidioschyzon merolae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ammonium + oxidized ferredoxin + H2O | - |
Cyanidioschyzon merolae | nitrite + reduced ferredoxin + H+ | - |
? | |
| hydrogen sulfide + oxidized ferredoxin + H2O | - |
Cyanidioschyzon merolae | sulfite + reduced ferredoxin + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMG021C | - |
Cyanidioschyzon merolae |
| CmSiRB | - |
Cyanidioschyzon merolae |
| ferredoxin-dependent sulfite reductase | - |
Cyanidioschyzon merolae |
| SIR | - |
Cyanidioschyzon merolae |
| sulfite reductase B | - |
Cyanidioschyzon merolae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Cyanidioschyzon merolae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
sulfite | - |
Cyanidioschyzon merolae | |
| 243 | - |
nitrite | - |
Cyanidioschyzon merolae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
activity assay | Cyanidioschyzon merolae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Cyanidioschyzon merolae | |
| iron-sulfur centre | - |
Cyanidioschyzon merolae | |
| siroheme | - |
Cyanidioschyzon merolae | |
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Release 2023.1 (January 2023)
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