Application | Comment | Organism |
---|---|---|
medicine | significant association with the age-at-onset of Alzheimer's disease and Parkinson's disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A140D | no alteration in specific activity compared to the wild type enzyme | Homo sapiens |
E155 | the deletion causes a 2-3fold increase in the specific activity with each substrate and a significant decrease in the enzyme's heat stability, it is also linked to abnormal arsenic excretion patterns | Homo sapiens |
E208K | no alteration in specific activity compared to the wild type enzyme | Homo sapiens |
N142D | no effect on the specific activity of the enzyme with any substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
dehydroascorbate | allelic variant D142 of GSTO2-2 | Homo sapiens | |
0.5 | - |
dehydroascorbate | allelic variant N142 of GSTO2-2 | Homo sapiens | |
11.4 | - |
glutathione | allelic variant D142 of GSTO2-2 | Homo sapiens | |
11.8 | - |
glutathione | allelic variant N142 of GSTO2-2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P78417 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-agarose affinity chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
GSTO2-2, using 8 mM urea | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
allelic variant A140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C | Homo sapiens |
0.14 | - |
allelic variant A140/E155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C | Homo sapiens |
0.14 | - |
allelic variant D140/E155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C | Homo sapiens |
0.21 | - |
allelic variant D140/DELTAE155/K208 of GSTO1-1, dehydroascorbate as substrate, at 30°C | Homo sapiens |
0.25 | - |
allelic variant A140/DELTAE155/E208 of GSTO1-1, dehydroascorbate as substrate, at 30°C | Homo sapiens |
11.6 | - |
allelic variant D142 of GSTO2-2, dehydroascorbate as substrate, at 30°C | Homo sapiens |
13.8 | - |
allelic variant N142 of GSTO2-2, dehydroascorbate as substrate, at 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Homo sapiens | GSSG + ascorbate | - |
? | |
glutathione + dehydroascorbate | - |
Homo sapiens | glutathione disulfide + ascorbate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Homo sapiens |
DHA reductase | - |
Homo sapiens |
DHAR | - |
Homo sapiens |
GSTO1-1 | - |
Homo sapiens |
GSTO2-2 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
at 53°C, both GSTO2-2 isoforms show a similar loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Homo sapiens |