Cloned (Comment) | Organism |
---|---|
a heterologous system is constructed in which the C domain of EiAPR was fused to the carboxyl terminus of the APS reductase from Pseudomonas aeruginosa (PaAPR), an enzyme that normally uses thioredoxin as an electron donor and is incapable of using glutathione for this function. The hybrid enzyme, which retains the [4Fe-4S] cluster from PaAPR can use both thioredoxin and glutathione as an electron donor for 5'-adenylylsulfate reduction. Expression in Escherichia coli | Ulva intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
glutathione | - |
Ulva intestinalis | |
0.011 | - |
5'-adenylyl sulfate | - |
Ulva intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ulva intestinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ulva intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + glutathione | - |
Ulva intestinalis | AMP + sulfite + glutathione disulfide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EiAPR | - |
Ulva intestinalis |