Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in wild-type plants | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
recombinant expression of the enzyme, fused to the transit peptide rbcS, in Arabidopsis thaliana under transcriptional control of the CaMV 35S promotor | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview | Arabidopsis thaliana |
additional information | construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
enzyme is highly stable in vitro | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + glutathione | Arabidopsis thaliana | enzyme is involved in reductive sulfate assimilation, pathway overview | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + glutathione | Pseudomonas aeruginosa | enzyme is involved in reductive sulfate assimilation, pathway overview | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | Pseudomonas aeruginosa | recombinant enzyme in transgenic Arabidopsis thaliana plants | AMP + sulfite + thioredoxin disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00077 | - |
wild-type plants | Arabidopsis thaliana |
0.0023 | 0.006 | recombinant enzyme in transgenic Arabidopsis thaliana plants, cofactor thioredoxin | Pseudomonas aeruginosa |
0.0023 | 0.006 | transgenic plants with the recombinant Pseudomonas aeruginosa enzyme utilizing thioredoxin | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + glutathione | - |
Arabidopsis thaliana | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + glutathione | - |
Pseudomonas aeruginosa | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + glutathione | enzyme is involved in reductive sulfate assimilation, pathway overview | Arabidopsis thaliana | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + glutathione | enzyme is involved in reductive sulfate assimilation, pathway overview | Pseudomonas aeruginosa | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylyl sulfate + thioredoxin | recombinant enzyme in transgenic Arabidopsis thaliana plants | Pseudomonas aeruginosa | AMP + sulfite + thioredoxin disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
5'-adenylylsulfate reductase | - |
Arabidopsis thaliana |
5'-adenylylsulfate reductase | - |
Pseudomonas aeruginosa |
APR | - |
Arabidopsis thaliana |
APR | - |
Pseudomonas aeruginosa |
APS reductase | - |
Arabidopsis thaliana |
APS reductase | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Arabidopsis thaliana | |
glutathione | - |
Pseudomonas aeruginosa | |
additional information | no activity with thioredoxin | Arabidopsis thaliana | |
thioredoxin | recombinant enzyme utilizes the plant thioredoxins m and f in transgenic plants | Pseudomonas aeruginosa |