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Literature summary for 1.8.4.9 extracted from

  • Tsakraklides, G.; Martin, M.; Chalam, R.; Tarczynski, M.C.; Schmidt, A.; Leustek, T.
    Sulfate reduction is increased in transgenic Arabidopsis thaliana expressing 5'-adenylylsulfate reductase from Pseudomonas aeruginosa (2002), Plant J., 32, 879-889.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in wild-type plants Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the enzyme, fused to the transit peptide rbcS, in Arabidopsis thaliana under transcriptional control of the CaMV 35S promotor Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview Arabidopsis thaliana
additional information construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview Pseudomonas aeruginosa

General Stability

General Stability Organism
enzyme is highly stable in vitro Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-adenylyl sulfate + glutathione Arabidopsis thaliana enzyme is involved in reductive sulfate assimilation, pathway overview AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + glutathione Pseudomonas aeruginosa enzyme is involved in reductive sulfate assimilation, pathway overview AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + thioredoxin Pseudomonas aeruginosa recombinant enzyme in transgenic Arabidopsis thaliana plants AMP + sulfite + thioredoxin disulfide
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00077
-
wild-type plants Arabidopsis thaliana
0.0023 0.006 recombinant enzyme in transgenic Arabidopsis thaliana plants, cofactor thioredoxin Pseudomonas aeruginosa
0.0023 0.006 transgenic plants with the recombinant Pseudomonas aeruginosa enzyme utilizing thioredoxin Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylyl sulfate + glutathione
-
Arabidopsis thaliana AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + glutathione
-
Pseudomonas aeruginosa AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + glutathione enzyme is involved in reductive sulfate assimilation, pathway overview Arabidopsis thaliana AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + glutathione enzyme is involved in reductive sulfate assimilation, pathway overview Pseudomonas aeruginosa AMP + sulfite + glutathione disulfide
-
?
5'-adenylyl sulfate + thioredoxin recombinant enzyme in transgenic Arabidopsis thaliana plants Pseudomonas aeruginosa AMP + sulfite + thioredoxin disulfide
-
?

Subunits

Subunits Comment Organism
? x * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
5'-adenylylsulfate reductase
-
Arabidopsis thaliana
5'-adenylylsulfate reductase
-
Pseudomonas aeruginosa
APR
-
Arabidopsis thaliana
APR
-
Pseudomonas aeruginosa
APS reductase
-
Arabidopsis thaliana
APS reductase
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Arabidopsis thaliana
glutathione
-
Pseudomonas aeruginosa
additional information no activity with thioredoxin Arabidopsis thaliana
thioredoxin recombinant enzyme utilizes the plant thioredoxins m and f in transgenic plants Pseudomonas aeruginosa