Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfate | optimal concentration between 350 and 500 mM | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
- |
Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
5'-adenylylsulfate | with glutathione as reductant | Catharanthus roseus | |
0.0038 | - |
5'-adenylylsulfate | with DTT as reductant | Catharanthus roseus | |
0.4 | - |
DTT | with 5'-adenylylsulfate as substrate | Catharanthus roseus | |
3 | - |
glutathione | with 5'-adenylylsulfate as substrate | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | precursor imported in pea chloroplast in vitro | Catharanthus roseus | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
mature protein without chloroplast transit peptide, calculated from cDNA sequence | Catharanthus roseus |
51390 | - |
calculated from cDNA sequence | Catharanthus roseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylylsulfate + dithioerythritol | - |
Catharanthus roseus | adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol | - |
? | |
5'-adenylylsulfate + dithiothreitol | - |
Catharanthus roseus | adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol | - |
? | |
5'-adenylylsulfate + glutathione | - |
Catharanthus roseus | adenosine monophosphate + sulfite + oxidized glutathione | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
5'-adenylylsulfate | with glutathione as reductant | Catharanthus roseus | |
3.4 | - |
5'-adenylylsulfate | with DTT as reductant | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
recombinant protein, expressed in Escherichia coli | Catharanthus roseus |