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Literature summary for 1.8.4.9 extracted from

  • Setya, A.; Murillo, M.; Leustek, T.
    Sulfate reduction in higher plants: Molecular evidence for a novel 5'-adenylylsulfate reductase (1996), Proc. Natl. Acad. Sci. USA, 93, 13383-13388.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.9

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 less effective than Na2SO4 Arabidopsis thaliana
DTT DTT can replace glutathione as reductant, 5 mM DTT increases activity 40fold Arabidopsis thaliana
Na2SO4
-
 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-phosphate
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast sequence contains chloroplast transit peptide Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45600
-
 clone APR2, calculated from cDNA sequence Arabidopsis thaliana
47300
-
 clone APR3, calculated from cDNA sequence Arabidopsis thaliana
50800
-
 clone APR1, calculated from cDNA sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000007
-
 with 3'-phosphoadenosine 5'-phosphosulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana
0.00022
-
 with 5'-adenylylsulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine-5'-phosphosulfate + glutathione 50fold lower activity than with 5'-adenylylsulfate Arabidopsis thaliana adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
 ?
5'-adenylylsulfate + dithiothreitol
-
 Arabidopsis thaliana adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
 ?
5'-adenylylsulfate + glutathione
-
 Arabidopsis thaliana adenosine monophosphate + sulfite + oxidized glutathione
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 recombinant protein, expressed in Escherichia coli Arabidopsis thaliana