Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | less effective than Na2SO4 | Arabidopsis thaliana | |
DTT | DTT can replace glutathione as reductant, 5 mM DTT increases activity 40fold | Arabidopsis thaliana | |
Na2SO4 | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-5'-phosphate | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | sequence contains chloroplast transit peptide | Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45600 | - |
clone APR2, calculated from cDNA sequence | Arabidopsis thaliana |
47300 | - |
clone APR3, calculated from cDNA sequence | Arabidopsis thaliana |
50800 | - |
clone APR1, calculated from cDNA sequence | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000007 | - |
with 3'-phosphoadenosine 5'-phosphosulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli | Arabidopsis thaliana |
0.00022 | - |
with 5'-adenylylsulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine-5'-phosphosulfate + glutathione | 50fold lower activity than with 5'-adenylylsulfate | Arabidopsis thaliana | adenosine 3',5'-diphosphate + sulfite + oxidized glutathione | - |
? | |
5'-adenylylsulfate + dithiothreitol | - |
Arabidopsis thaliana | adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol | - |
? | |
5'-adenylylsulfate + glutathione | - |
Arabidopsis thaliana | adenosine monophosphate + sulfite + oxidized glutathione | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant protein, expressed in Escherichia coli | Arabidopsis thaliana |