Any feedback?
Literature summary for 1.8.4.8 extracted from
- Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis (2004), J. Biol. Chem., 279, 7850-7855.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cysH, expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| activity is lost upon exposure to air or upon dilution | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis |  |
| 0.0107 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
| 0.0225 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
| 0.0287 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
| 0.0543 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
| 0.0606 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
| 0.0675 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
| 0.1054 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains a [4Fe-4S] cluster per subunit, absolutely required for activity, constitution is altered in presence of oxygen, activity is restored after decomposition and reassembly | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-adenylyl-sulfate + thioredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
| 3'-phosphoadenylyl-sulfate + glutaredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
| 3'-phosphoadenylyl-sulfate + thioredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene cysH, bispecific enzyme | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| activity is lost upon exposure to air | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-adenylyl-sulfate + thioredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
| 3'-phosphoadenylyl sulfate + glutaredoxin 1 | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide | - |
r | |
| 3'-phosphoadenylyl sulfate + thioredoxin 1 | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide | - |
r | |
| 3'-phosphoadenylyl-sulfate + glutaredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
| 3'-phosphoadenylyl-sulfate + thioredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase | - |
Bacillus subtilis |
| CysH1 | - |
Bacillus subtilis |
| SNR | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.146 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
| 0.149 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
| 0.172 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
| 0.174 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
| 0.219 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
| 0.335 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
| 0.382 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
| 0.421 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutaredoxin | glutaredoxin 1 variant | Bacillus subtilis | |
| thioredoxin | thioredoxin 1 variant | Bacillus subtilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
