Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.8 extracted from

  • Berendt, U.; Haverkamp, T.; Prior, A.; Schwenn, J.D.
    Reaction mechanism of thioredoxin:3'-phospho-adenylylsulfate reductase investigated by site-directed mutagenesis (1995), Eur. J. Biochem., 233, 347-356.
    View publication on PubMed
Search for more literature for 1.8.4.8

Protein Variants

Protein Variants Comment Organism
C239S mutant enzymes Cys239Ser and Tyr209Phe are inactive Escherichia coli
Q160L mutant enzyme Gln160Leu is as active as the wild-type enzyme Escherichia coli
Y209F mutant enzymes Cys239Ser and Tyr209Phe are inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate competitive with respect to 5-phosphoadenosine 3-phosphosulfate Escherichia coli
N-(Iodoacetyl-aminoethyl)-5-N'-naphthylamin-1-sulfonic acid
-
 Escherichia coli
N-bromosuccinimide
-
 Escherichia coli
p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 5-Phosphoadenosine 3-phosphosulfate
-
 Escherichia coli
0.023
-
 reduced thioredoxin
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 recombinant enzyme
-

Reaction

Reaction Comment Organism Reaction ID
adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin special ping-pong mechanism with 5-phosphoadenosine 3-phosphosulfate reacting with the reduced enzyme isomer in a Theorell-Chance mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
99
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin
-
 Escherichia coli adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite
-
 ?