Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli from plasmid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | oxidation activity | Escherichia coli | |
guanidinium hydrochloride | destroys zinc finger thiols of DnaJ protein | Escherichia coli | |
additional information | carboxylation of thiol groups abolish reduction activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | essential for activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
thiol-disulfide oxidoreductase like activity from gene DnaJ | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity | Escherichia coli | |
2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | zinc finger protein with several CXXC motifs | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
reductase activity after several treatments | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + insulin | - |
Escherichia coli | GSSG + reduced insulin chain A and B | - |
? | |
GSSG + reduced ribonuclease | - |
Escherichia coli | GSH + oxidized ribonuclease | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Escherichia coli |