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Literature summary for 1.8.4.2 extracted from

  • Tang, W.; Wang, C.C.
    Zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ (2001), Biochemistry, 40, 14985-14994.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli from plasmid Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA oxidation activity Escherichia coli
guanidinium hydrochloride destroys zinc finger thiols of DnaJ protein Escherichia coli
additional information carboxylation of thiol groups abolish reduction activity Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential for activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
thiol-disulfide oxidoreductase like activity from gene DnaJ
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity Escherichia coli
2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol zinc finger protein with several CXXC motifs Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
reductase activity after several treatments Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSH + insulin
-
Escherichia coli GSSG + reduced insulin chain A and B
-
?
GSSG + reduced ribonuclease
-
Escherichia coli GSH + oxidized ribonuclease
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Escherichia coli