Application | Comment | Organism |
---|---|---|
medicine | DsbA protein is a target for treatment of pathogenic bacteria | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | DsbA and DsbB insertion mutants are sensitive to dithiothreitol and benzylpenicillin or Cd2+, Hg2+ and Zn2+, pleitropic phenotype | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | outer cytoplasmic membrane, protein termini localised in the periplasm | Escherichia coli | 16020 | - |
periplasm | DsbA protein soluble, DsbB protein is membrane intrinsic | Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
DsbB gene product | Escherichia coli |
21100 | - |
DsbA gene product | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + protein disulfide | Escherichia coli | disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds | GSSG + protein-dithiol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
DsbA homologous | - |
Dickeya chrysanthemi DsbA homologous | - |
DsbA homologous | - |
Escherichia coli | - |
in periplasm: DsbA and DsbB enzymes | - |
Escherichia coli | - |
different forms of protein thiol:disulfide oxidoreductases with either oxidizing, gene DsbA, reducing, gene DsbB, or isomerising, gene DsbC and DsbD, activity exist in Escherichia coli | - |
Haemophilus influenzae | - |
DsbA homologous | - |
Haemophilus influenzae DsbA homologous | - |
DsbA homologous | - |
Klebsiella sp. | - |
DsbA homologous | - |
Klebsiella sp. DsbA homologous | - |
DsbA homologous | - |
Legionella pneumophila | P50024 | DsbA homologous | - |
Legionella pneumophila DsbA homologous | P50024 | DsbA homologous | - |
Lelliottia amnigena | Q9XDP0 | DsbA homologous | - |
Lelliottia amnigena DsbA homologous | Q9XDP0 | DsbA homologous | - |
Pectobacterium carotovorum | - |
DsbA homologous | - |
Pectobacterium carotovorum DsbA homologous | - |
DsbA homologous | - |
Pseudomonas aeruginosa | P0C2B2 | DsbA homologous | - |
Pseudomonas aeruginosa DsbA homologous | P0C2B2 | DsbA homologous | - |
Pseudomonas syringae | O52376 | DsbA homologous | - |
Pseudomonas syringae DsbA homologous | O52376 | DsbA homologous | - |
Salmonella enterica subsp. enterica serovar Enteritidis | - |
DsbA homologous | - |
Salmonella enterica subsp. enterica serovar Enteritidis DsbA homologous | - |
DsbA homologous | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
DsbA homologous | - |
Salmonella enterica subsp. enterica serovar Typhimurium DsbA homologous | - |
DsbA homologous | - |
Shigella flexneri | - |
DsbA homologous | - |
Shigella flexneri DsbA homologous | - |
DsbA homologous | - |
Vibrio cholerae serotype O1 | - |
DsbA homologous | - |
Vibrio cholerae serotype O1 DsbA homologous | - |
DsbA homologous | - |
Yersinia pestis | - |
DsbA homologous | - |
Yersinia pestis DsbA homologous | - |
DsbA homologous | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + protein disulfide | very rapid disulfide interchange reaction | Escherichia coli | GSSG + protein-dithiol | - |
r | |
GSH + protein disulfide | disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds | Escherichia coli | GSSG + protein-dithiol | - |
r | |
additional information | DsbB protein re-oxidizes the reduced DsbA protein | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21100 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | DsbA protein family including periplasmic protein disulfide: thiol oxidoreductases with high homology in amino acid sequences | Escherichia coli |