Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.14 extracted from

  • Kim, H.S.; Kwak, G.H.; Lee, K.; Jo, C.H.; Hwang, K.Y.; Kim, H.Y.
    Structural and biochemical analysis of a type II free methionine-R-sulfoxide reductase from Thermoplasma acidophilum (2014), Arch. Biochem. Biophys., 560, 10-9.
    View publication on PubMed
Search for more literature for 1.8.4.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method at 20°C. Crystal structure is determined in native form and in a complex with the substrate Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
C15S mutant exhibits apparent activity. The kcat and Km values of the mutant enzyme are similar to those of wild type, showing that Cys15 is dispensable for catalysis Thermoplasma acidophilum
C60S mutant exhibits apparent activity. The kcat value is dramatically reduced in the C60S mutant (80fold lower), while the Km value does not significantly change compared to wild type Thermoplasma acidophilum
C84S no detectable activity. Cells expressing C84S do not grow in the Met-O medium at all Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.4
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum
0.47
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-S-oxide + thioredoxin the enzyme only reduces the free form of methionine-R-sulfoxide Thermoplasma acidophilum L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine-R-sulfoxide reductase
-
 Thermoplasma acidophilum
TafRMsr
-
 Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.268
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum
0.29
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
0.62
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum
0.67
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum