KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
L-methionine (S)-S-oxide | pH 7.5, 37°C | Escherichia coli B |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli B |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, SDS-PAGE | Escherichia coli B |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli B | methionine sulfoxide reduction pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme about 1100fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again anion exchange chromatography, heat treatment, and hydroxyapatite chromatography to near homogeneity | Escherichia coli B |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00452 | - |
purified enzyme | Escherichia coli B |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-S-oxide + NADPH | - |
Escherichia coli B | L-methionine + NADP+ + H2O | - |
? | |
L-methionine (S)-S-oxide + reduced DTT | highest activity at 10 mM | Escherichia coli B | L-methionine + oxidized DTT + H2O | - |
? | |
additional information | methionine sulfoxide reduction pathway, overview | Escherichia coli B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, SDS-PAGE | Escherichia coli B |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Escherichia coli |
methionine sulfoxide reductase | - |
Escherichia coli B |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli B |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli B |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | required, can be substituted by DTT, but not by GSH or 2-mercaptoethanol | Escherichia coli B |