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Literature summary for 1.8.4.12 extracted from

  • Fukushima, E.; Shinka, Y.; Fukui, T.; Atomi, H.; Imanaka, T.
    Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures (2007), J. Bacteriol., 189, 7134-7144.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 dithiothreitol MsrB domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide Thermococcus kodakarensis
67
-
 L-methionine (R)-sulfoxide MsrB domain of MsrABTk, in the presence of 20 mM dithiothreiol Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information presence of MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures Thermococcus kodakarensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine-(R)-S-oxide + dithiothreitol
-
 Thermococcus kodakarensis dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + dithiothreitol the MsrB-domain of MsrABTk is strictly specific for the reduction of L-methionine (R)-sulfoxide Thermococcus kodakarensis L-methionine + dithiothreitol disulfide
-
 ?

Synonyms

Synonyms Comment Organism
MsrABTk bifunctional enzyme (EC 1.8.1.11/1.8.1.12) Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 MsrB domain of MsrABTk Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life of MsrABTk is 30 min, half-life of MsrB domain is 31 min Thermococcus kodakarensis
85
-
 75% decrease in activity after 2.5 min, MsrABTk Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 optimum pH of the MsrB domain Thermococcus kodakarensis