Literature summary for 1.8.4.12 extracted from

Weissbach, H.; Resnick, L.; Brot, N.
Methionine sulfoxide reductases: history and cellular role in protecting against oxidative damage (2005), Biochim. Biophys. Acta, 1703, 203-212.

Application

Application	Comment	Organism
synthesis	enzyme can be useful in the development and action of anti-cancer and anti-inflammation drugs	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
gene msr or pilB, DNA sequence determination and analysis	Neisseria gonorrhoeae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a msrA/msrB double mutant for detection of additional enzyme form activities	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	Sel-X, a MsrB enzyme form variant	Homo sapiens	5829	-
cytosol	Sel-X, a MsrB enzyme form variant	Saccharomyces cerevisiae	5829	-
cytosol	Sel-X, a MsrB enzyme form variant, sulindac reducing activity in calf	Bos taurus	5829	-
cytosol	Sel-X, a MsrB enzyme variant	Mus musculus	5829	-
membrane	enzyme form Mem-R,S-Msr	Escherichia coli	16020	-
microsome	calf, sulindac reducing activity	Bos taurus	-	-
mitochondrion	Sel-X, a MsrB enzyme form variant	Homo sapiens	5739	-
mitochondrion	Sel-X, a MsrB enzyme form variant	Saccharomyces cerevisiae	5739	-
mitochondrion	Sel-X, a MsrB enzyme form variant, sulindac reducing activity in calf	Bos taurus	5739	-
mitochondrion	Sel-X, a MsrB enzyme variant	Mus musculus	5739	-
additional information	subcellular sulindac reducing activity distribution in calf liver	Bos taurus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
selenium	selenoprotein	Saccharomyces cerevisiae
selenium	selenoprotein	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	x * 57000, MsrA/B	Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
L-methionine (R)-sulfoxide + thioredoxin	Bos taurus	enzyme form MsrB is specific for the R-form, enzyme form variants with specificities for either free or protein-bound methionine	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	Escherichia coli	membrane-bound enzyme form Mem-R,S-Msr, enzyme form MsrB is specific for the R-form, MsrB enzyme form variants with specificities for either free or protein-bound methionine, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	Mus musculus	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	Homo sapiens	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	Saccharomyces cerevisiae	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Haemophilus influenzae	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Neisseria gonorrhoeae	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Neisseria meningitidis	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Streptococcus pneumoniae	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Helicobacter pylori	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	Streptococcus gordonii	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	L-methionine + thioredoxin disulfide	-	?
additional information	Mus musculus	the enzyme protect cells against oxidative damage and plays a role in age-related diseases	?	-	?
additional information	Escherichia coli	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	?	-	?
additional information	Homo sapiens	the enzyme protects cells against oxidative damage and plays a role in age-related and neurological diseases, like Parkinsons or Alzheimers disease	?	-	?
additional information	Saccharomyces cerevisiae	the enzyme protects cells against oxidative damage and plays a role in age-related diseases	?	-	?
additional information	Bos taurus	the enzyme protects cells against oxidative damage and plays a role in age-related diseases	?	-	?
sulindac + thioredoxin	Bos taurus	activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr	sulindac sulfide + thioredoxin disulfide	activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity	?
sulindac + thioredoxin	Escherichia coli	activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA	sulindac sulfide + thioredoxin disulfide	activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	MsrB variants one of which is also called Sel-X	-
Escherichia coli	-	enzyme forms MsrB and Mem-R,S-Msr	-
Haemophilus influenzae	-	-	-
Haemophilus influenzae	-	gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity	-
Helicobacter pylori	-	-	-
Helicobacter pylori	-	gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity	-
Homo sapiens	-	MsrB variants one of which is also called Sel-X, encoded by distinct genes, MsrB is a selenoprotein	-
Mus musculus	-	MsrB is a selenoprotein	-
Neisseria gonorrhoeae	-	-	-
Neisseria gonorrhoeae	-	gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity	-
Neisseria meningitidis	-	-	-
Neisseria meningitidis	-	gene msr encodes an enzyme showing both MsrA, EC 1.8.4.B2, and MsrB activity	-
Saccharomyces cerevisiae	-	MsrB variants one of which is also called Sel-X	-
Streptococcus gordonii	-	gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity	-
Streptococcus gordonii	Q9LAM9	-	-
Streptococcus pneumoniae	-	-	-
Streptococcus pneumoniae	-	gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O	catalytic mechanism involving the formation of a sulfenic acid intermediate	Escherichia coli	a
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O	catalytic mechanism involving the formation of a sulfenic acid intermediate	Bos taurus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	calf, sulindac reducing activity	Bos taurus	-
kidney	calf, sulindac reducing activity	Bos taurus	-
liver	calf, sulindac reducing activity	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	subcellular sulindac reducing activity in calf liver	Bos taurus
0.00009	-	enzyme form Mem-R,S-Msr, substrate sulindac	Escherichia coli
0.0048	-	wild-type strain, substrate L-methionine (R)-sulfoxide	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-methionine (R)-sulfoxide + thioredoxin	enzyme form MsrB is specific for the R-form, enzyme form variants with specificities for either free or protein-bound methionine	Bos taurus	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	membrane-bound enzyme form Mem-R,S-Msr, enzyme form MsrB is specific for the R-form, MsrB enzyme form variants with specificities for either free or protein-bound methionine, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate	Escherichia coli	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	Mus musculus	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	Homo sapiens	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	Saccharomyces cerevisiae	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine	Bos taurus	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R)-sulfoxide + thioredoxin	membrane-bound enzyme form Mem-R,S-Msr	Escherichia coli	L-methionine + thioredoxin disulfide + H2O	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Haemophilus influenzae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Neisseria gonorrhoeae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Neisseria meningitidis	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Streptococcus pneumoniae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Helicobacter pylori	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Streptococcus gordonii	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Haemophilus influenzae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Neisseria gonorrhoeae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Neisseria meningitidis	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Streptococcus pneumoniae	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Helicobacter pylori	L-methionine + thioredoxin disulfide	-	?
L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Streptococcus gordonii	L-methionine + thioredoxin disulfide	-	?
L-methionine sulfoxide enkephalin + thioredoxin	membrane-bound enzyme form Mem-R,S-Msr	Escherichia coli	L-methionine enkephalin	-	?
additional information	the enzyme protect cells against oxidative damage and plays a role in age-related diseases	Mus musculus	?	-	?
additional information	the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions	Escherichia coli	?	-	?
additional information	the enzyme protects cells against oxidative damage and plays a role in age-related and neurological diseases, like Parkinsons or Alzheimers disease	Homo sapiens	?	-	?
additional information	the enzyme protects cells against oxidative damage and plays a role in age-related diseases	Saccharomyces cerevisiae	?	-	?
additional information	the enzyme protects cells against oxidative damage and plays a role in age-related diseases	Bos taurus	?	-	?
additional information	enzyme reduces oxidized methionine residues of the alpha-1-proteinase inhibitor, calmodulin, and thrombomodulin, which become reversibly inactivated upon oxidation	Homo sapiens	?	-	?
additional information	substrate specificity and activity of MsrB/PilB in comparison to MsrA, overview	Neisseria gonorrhoeae	?	-	?
additional information	substrate specificity of the different enzyme forms, overview, enzyme reduces oxidized methionine residues of the ribosomal protein L12, which becomes reversibly inactivated and forms monomers instead of dimers upon oxidation, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate	Escherichia coli	?	-	?
N-acetyl-L-methionine (R,S)-sulfoxide + thioredoxin	enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine	Neisseria gonorrhoeae	N-acetyl-L-methionine + thioredoxin disulfide	-	?
N-acetyl-L-methionine (R,S)-sulfoxide + thioredoxin	membrane-bound enzyme form Mem-R,S-Msr	Escherichia coli	N-acetyl-L-methionine + thioredoxin disulfide	-	?
sulindac + thioredoxin	activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr	Bos taurus	sulindac sulfide + thioredoxin disulfide	activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity	?
sulindac + thioredoxin	activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA	Escherichia coli	sulindac sulfide + thioredoxin disulfide	activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity	?
sulindac + thioredoxin	activity with membrane-bound enzyme form Mem-R,S-Msr	Bos taurus	sulindac sulfide + thioredoxin disulfide	-	?
sulindac + thioredoxin	activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA	Escherichia coli	sulindac sulfide + thioredoxin disulfide	-	?

Subunits

Subunits	Comment	Organism
?	x * 57000, MsrA/B	Neisseria gonorrhoeae

Synonyms

Synonyms	Comment	Organism
methionine sulfoxide reductase	-	Haemophilus influenzae
methionine sulfoxide reductase	-	Mus musculus
methionine sulfoxide reductase	-	Escherichia coli
methionine sulfoxide reductase	-	Homo sapiens
methionine sulfoxide reductase	-	Saccharomyces cerevisiae
methionine sulfoxide reductase	-	Bos taurus
methionine sulfoxide reductase	-	Neisseria gonorrhoeae
methionine sulfoxide reductase	-	Neisseria meningitidis
methionine sulfoxide reductase	-	Streptococcus pneumoniae
methionine sulfoxide reductase	-	Helicobacter pylori
methionine sulfoxide reductase	-	Streptococcus gordonii
MSR	-	Haemophilus influenzae
MSR	-	Escherichia coli
MSR	-	Neisseria meningitidis
MSR	-	Streptococcus pneumoniae
MSR	-	Helicobacter pylori
MSR	-	Streptococcus gordonii
MsrA/B	-	Neisseria gonorrhoeae
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Haemophilus influenzae
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Neisseria gonorrhoeae
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Neisseria meningitidis
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Streptococcus pneumoniae
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Helicobacter pylori
MsrA/B	bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12	Streptococcus gordonii
MsrB	-	Mus musculus
MsrB	-	Escherichia coli
MsrB	-	Homo sapiens
MsrB	-	Saccharomyces cerevisiae
MsrB	-	Bos taurus
PilB	-	Neisseria gonorrhoeae
Sel-X	-	Mus musculus
Sel-X	-	Homo sapiens
Sel-X	-	Saccharomyces cerevisiae
Sel-X	-	Bos taurus

Cofactor

Cofactor	Comment	Organism
additional information	no activity with DTT as cofactor by membrane-bound enzyme form Mem-R,S-Msr	Escherichia coli
NADPH	membrane-bound enzyme form Mem-R,S-Msr	Escherichia coli
thioredoxin	-	Haemophilus influenzae
thioredoxin	-	Mus musculus
thioredoxin	-	Homo sapiens
thioredoxin	-	Saccharomyces cerevisiae
thioredoxin	-	Bos taurus
thioredoxin	-	Neisseria gonorrhoeae
thioredoxin	-	Neisseria meningitidis
thioredoxin	-	Streptococcus pneumoniae
thioredoxin	-	Helicobacter pylori
thioredoxin	-	Streptococcus gordonii
thioredoxin	preferred cofactor	Escherichia coli