Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) fused to a 6His-MBP | Leptospira interrogans |
expression in Escherichia coli BL21 (DE3) fused to an N-terminal 6Histag | Leptospira interrogans |
Protein Variants | Comment | Organism |
---|---|---|
C18S | exhibits lower enzymatic activity than the wild type enzyme | Leptospira interrogans |
C25S | exhibits lower enzymatic activity than the wild type enzyme | Leptospira interrogans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.002 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.019 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.0249 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.0305 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.135 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.28 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.39 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.55 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
1.4 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | dual localization, both in cytoplasm and periplasm | Leptospira interrogans | 5737 | - |
periplasm | dual localization, both in cytoplasm and periplasm | Leptospira interrogans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
calculated from sequence | Leptospira interrogans |
23000 | - |
calculated from sequence | Leptospira interrogans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans | Q72N60 | serovar Copenhageni | - |
Leptospira interrogans | Q72UW2 | serovar Copenhageni | - |
Leptospira interrogans Fiocruz L1-130 | Q72N60 | serovar Copenhageni | - |
Leptospira interrogans Fiocruz L1-130 | Q72UW2 | serovar Copenhageni | - |
Purification (Comment) | Organism |
---|---|
- |
Leptospira interrogans |
Storage Stability | Organism |
---|---|
-80°C, 8 months, no loss of activity | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine-(S)-S-oxide + thioredoxin | thioredoxin from Leptospira interrogans | Leptospira interrogans | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine-(S)-S-oxide + thioredoxin | thioredoxin from Leptospira interrogans | Leptospira interrogans Fiocruz L1-130 | L-methionine + thioredoxin disulfide + H2O | - |
? | |
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin | thioredoxin from Leptospira interrogans | Leptospira interrogans | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin | thioredoxin from Leptospira interrogans | Leptospira interrogans Fiocruz L1-130 | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LIC_10545 | - |
Leptospira interrogans |
LIC_12978 | - |
Leptospira interrogans |
linmsra1 | - |
Leptospira interrogans |
linmsra2 | - |
Leptospira interrogans |
MsrA1 | - |
Leptospira interrogans |
MSRA2 | - |
Leptospira interrogans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.0548 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.083 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.098 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.102 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.203 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.32 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
0.39 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.415 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.508 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.04 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S | Leptospira interrogans | |
0.14 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.15 | - |
N-acetyl-L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.25 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
0.71 | - |
L-methionine-(S)-S-oxide | 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme | Leptospira interrogans | |
40 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
42 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
50 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans | |
56 | - |
thioredoxin | 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme | Leptospira interrogans |