Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
C180S | the mutant of isoform E4 shows wild type activity | Solanum lycopersicum |
C180S | the mutant of isoform MSRA2 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
C188S | the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
C194S | the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
C37S | inactive | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00322 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.00343 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.0189 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.0218 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.02265 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.0233 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, SDS-PAGE | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Solanum lycopersicum | - |
L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | G3JX11 | cultivar Micro-Tom | - |
Solanum lycopersicum | P54153 | cultivar Micro-Tom | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol | - |
Solanum lycopersicum | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | - |
Solanum lycopersicum | L-methionine + thioredoxin disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase A | - |
Solanum lycopersicum |
MSRA2 | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.1 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.11 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.13 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.15 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
0.17 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Solanum lycopersicum |
Organism | Comment | Expression |
---|---|---|
Solanum lycopersicum | the transcript level of isoform MSRA2 is significantly accumulated in both leaves and green fruits after ethephon treatment | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
4.9 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
5.1 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
6.9 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
46.5 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
49.5 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |