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Literature summary for 1.8.4.11 extracted from

  • Kim, H.Y.; Zhang, Y.; Lee, B.C.; Kim, J.R.; Gladyshev, V.N.
    The selenoproteome of Clostridium sp. OhILAs: characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase A (2009), Proteins, 74, 1008-1017.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium sp.

Protein Variants

Protein Variants Comment Organism
additional information the cysteine mutant form U16C exhibits at least a 20fold lower activity than the selenocysteine-containing wild type MsrA enzyme Clostridium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 dabsylated L-methionine (S)-sulfoxide purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
10.2
-
 dabsylated L-methionine (S)-sulfoxide wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
selenium selenoprotein, has a single selenocysteine residue at the catalytic site, the selenocysteine-containing MsrA exhibits at least a 20fold higher activity than its cysteine mutant form U16C, indicating a critical role of selenocysteine in the catalytic activity of the enzyme Clostridium sp.

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
 also known as Alkaliphilus oremlandii
-
Clostridium sp. OhILAs
-
 also known as Alkaliphilus oremlandii
-

Purification (Commentary)

Purification (Comment) Organism
Talon-metal affinity resin chromatography Clostridium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
 purified cysteine mutant form U16C, using human thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
0.023
-
 purified cysteine mutant form U16C, using thioredoxin from Escherichia coli as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
0.034
-
 purified wild type enzyme, using human thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
0.07
-
 purified wild type enzyme, using Escherichia coli thioredoxin as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
0.464
-
 purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
4.185
-
 purified wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Clostridium sp. dabsyl-L-methionine + dithiothreitol disulfide
-
 ?
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Clostridium sp. OhILAs dabsyl-L-methionine + dithiothreitol disulfide
-
 ?
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. dabsyl-L-methionine + thioredoxin disulfide
-
 ?
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. OhILAs dabsyl-L-methionine + thioredoxin disulfide
-
 ?
dabsylated L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. dabsylated L-methionine + thioredoxin disulfide + H2O
-
 r
dabsylated L-methionine (S)-sulfoxide + thioredoxin
-
 Clostridium sp. OhILAs dabsylated L-methionine + thioredoxin disulfide + H2O
-
 r

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase A
-
 Clostridium sp.
MsrA
-
 Clostridium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 dabsylated L-methionine (S)-sulfoxide purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.
84
-
 dabsylated L-methionine (S)-sulfoxide wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C Clostridium sp.

Cofactor

Cofactor Comment Organism Structure
dithiothreitol
-
 Clostridium sp.
thioredoxin Clostridium MsrA is inefficiently reducible by thioredoxin Clostridium sp.