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Literature summary for 1.8.4.11 extracted from

  • Fukushima, E.; Shinka, Y.; Fukui, T.; Atomi, H.; Imanaka, T.
    Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures (2007), J. Bacteriol., 189, 7134-7144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
dithiothreitol MsrA domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide Thermococcus kodakarensis
19
-
L-methionine (S)-sulfoxide MsrA domain of MsrABTk, in the presence of 100 mM dithiothreiol Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
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-
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Source Tissue

Source Tissue Comment Organism Textmining
additional information presence o MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures Thermococcus kodakarensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine-(S)-S-oxide + dithiothreitol
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Thermococcus kodakarensis dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine (S)-sulfoxide + 2 dithiothreitol the MsrA-domain of MsrABTk is strictly specific for the reduction of L-methionine (S)-sulfoxide Thermococcus kodakarensis L-methionine + dithiothreitol disulfide + H2O
-
?

Synonyms

Synonyms Comment Organism
MsrABTk bifunctional enzyme (EC 1.8.1.11/1.8.1.12) Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
MsrA domain of MsrABTk, substrate: dabsyl-L-methionine-(S)-S-oxide Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life of MsrABTk is 30 min. Half-life of the MsrA domain within the MsrABTk protein is 27 min. The recombinant MsrA protein shows a half-life of less than 1 min. The MsrA domain is stabilized through its physical interaction with the more thermostable MsrB domain Thermococcus kodakarensis
85
-
75% decrease in activity after 2.5 min, MsrABTk Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 optimum pH of the MsrA domain Thermococcus kodakarensis