Cloned (Comment) | Organism |
---|---|
overexpression of N-terminally His-tagged wild-type and mutant MsrAs in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
C107S | site-directed mutagenesis, the mutant shows 14% increased activity with DTT and 4% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C107S/C218S | site-directed mutagenesis, the mutant shows 78% reduced activity with DTT and 94% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C107S/C218S/C227S | site-directed mutagenesis, the mutant shows 61% reduced activity with DTT and 92% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C107S/C227S | site-directed mutagenesis, the mutant shows 4% reduced activity with DTT and 86% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C218S | site-directed mutagenesis, the mutant shows 65% reduced activity with DTT and 78% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C218S/C227S | site-directed mutagenesis, the mutant shows 58% reduced activity with DTT and 96% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C227S | site-directed mutagenesis, the mutant shows 11% reduced activity with DTT and 81% with thioredoxin compared to the wild-type enzyme | Bos taurus |
C72S | site-directed mutagenesis, inactive mutant, no disulfide bond in the mutant enzyme | Bos taurus |
C72S/C107S/C227S | site-directed mutagenesis, inactuve mutant | Bos taurus |
C72S/C218S | site-directed mutagenesis, inactive mutant | Bos taurus |
additional information | free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require meal ions for activity, free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and mutant MsrAs from Escherichia coli by nickel affinity chromatography and dialysis | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin | catalytic mechanism, Cys72 is essential for activity forming disulfide bonds with either Cys218 or Cys227 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-methionine-(S)-S-oxide + DTT | stereospecific reduction | Bos taurus | N-acetyl-L-methionine + DTT disulfide + H2O | - |
? | |
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Bos taurus | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Bos taurus |
peptide methionine sulfoxide reductase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Bos taurus | |
thioredoxin | - |
Bos taurus |