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Literature summary for 1.8.4.10 extracted from

  • Prior, A.; Uhrig, J.F.; Heins, L.; Wiesmann, A.; Lillig, C.H.; Stoltze, C.; Soll, J.; Schwenn, J.D.
    Structural and kinetic properties of adenylyl sulfate reductase from Catharanthus roseus cell cultures (1999), Biochim. Biophys. Acta, 1430, 25-38.
    View publication on PubMed
Search for more literature for 1.8.4.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes lacking transit sequence or transit sequence and C-terminal domain in Escherichia coli Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductand glutathione Catharanthus roseus
0.0038
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductand dithiothreitol Catharanthus roseus
0.4
-
 reduced dithiothreitol recombinant enzyme lacking transit peptide Catharanthus roseus
3
-
 glutathione recombinant enzyme lacking transit peptide Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51394
-
 x * 51394, preprotein, deduced from nucleotide sequence Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus Q39619
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme, metal affinity resin Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylyl sulfate + glutathione enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown Catharanthus roseus AMP + sulfite + glutathione disulfide
-
 ?
5'-adenylyl sulfate + reduced dithiothreitol enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown Catharanthus roseus AMP + sulfite + oxidized dithiothreitol
-
 ?

Subunits

Subunits Comment Organism
? x * 51394, preprotein, deduced from nucleotide sequence Catharanthus roseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductant glutathione Catharanthus roseus
3.4
-
 adenosine-5'-phosphosulfate recombinant enzyme lacking transit peptide, artificial reductant dithiothreitol Catharanthus roseus