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Literature summary for 1.8.3.5 extracted from

  • Crowell, D.N.; Huizinga, D.H.; Deem, A.K.; Trobaugh, C.; Denton, R.; Sen, S.E.
    Arabidopsis thaliana plants possess a specific farnesylcysteine lyase that is involved in detoxification and recycling of farnesylcysteine (2007), Plant J., 50, 839-847.
    View publication on PubMed
Search for more literature for 1.8.3.5

Cloned(Commentary)

Cloned (Comment) Organism
gene FCLY, encoded on chromosome 5, DNAS and amino acid sequence determination and analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana Col-0 the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P57681 gene FCLY
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Arabidopsis thaliana Col-0 P57681 gene FCLY
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
FC lyase
-
 Arabidopsis thaliana
FCLY
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acidsensitivity are even greater in the presence of exogenous S-(2E,6E)-farnesyl-L-cysteine Arabidopsis thaliana
metabolism the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview Arabidopsis thaliana
physiological function Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins Arabidopsis thaliana