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Literature summary for 1.8.3.2 extracted from

  • Moubasher, H.; Fahmi, A.; Abdur-Rahman, M.
    Induction, purification and molecular characterization of sulfhydryl oxidase from an Egyptian isolates of Aspergillus niger (2012), Appl. Biochem. Microbiol., 48, 290-294.
No PubMed abstract available
Search for more literature for 1.8.3.2

Activating Compound

Activating Compound Comment Organism Structure
L-Cys fungus is not able to use L-Cys as sulfur source instead of sulfate. Presence of L-Cys induces production of an excessive amount of both intracellular and extracellular enzyme Aspergillus niger
MgSO4 in the absence of MgSO4 in the growth medium, growth is weak and no enzyme activity detected. Highest activity at 0.1% MgSO4 in the medium Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
 glutathione pH 5.5, 25°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular both intra- and extracellular Aspergillus niger
-
-
intracellular both intra- and extracellular Aspergillus niger 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgSO4 in the absence of MgSO4 in the growth medium, growth is weak and no enzyme activity detected. Highest activity at 0.1% MgSO4 in the medium Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger
55000
-
 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger AUMC 4947
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + O2
-
 Aspergillus niger glutathione disulfide + H2O2
-
 ?
2 glutathione + O2
-
 Aspergillus niger AUMC 4947 glutathione disulfide + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus niger