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Literature summary for 1.8.3.2 extracted from

  • Takamori, K.; Thorpe, J.M.; Goldsmith, L.A.
    Skin sulfhydryl oxidase. Purification and some properties (1980), Biochim. Biophys. Acta, 615, 309-323.
    View publication on PubMed
Search for more literature for 1.8.3.2

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0-0.1% concentration, at most 30% activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide moderate Rattus norvegicus
iodoacetic acid moderate Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.515
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, stable for at least 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol + O2 3.7% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + O2
-
 Rattus norvegicus ? + H2O
-
 ?
D-penicillamine + O2 33% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
 ?
dithioerythritol + O2
-
 Rattus norvegicus ? + H2O
-
 ?
dithiothreitol + O2
-
 Rattus norvegicus ? + H2O
-
 ?
GSH + O2 + O2 0.7% of the activity with dithiothreitol Rattus norvegicus GSSG + H2O
-
 ?
L-Cys + O2 17% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
 ?
N-acetylcysteine + O2 4.6% of the activity with dithiothreitol Rattus norvegicus ? + H2O
-
 ?
reductively denatured ribonuclease A + O2
-
 Rattus norvegicus renatured ribonuclease + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 dithiothreitol, 1 mM, increases the stability to heating Rattus norvegicus
60
-
 15 min, 85% loss of activity. 30 min, complete loss of activity Rattus norvegicus