Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F57A | the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate | Homo sapiens |
F57Y | the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate | Homo sapiens |
F79A | the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate | Homo sapiens |
H90F | interactions of H90 with a heme propionate group destabilize the Fe(III) state of the heme | Homo sapiens |
H90Y | interactions of H90 with a heme propionate group destabilize the Fe(III) state of the heme | Homo sapiens |
Y83A | mutation is located on the surface of the heme domain, but not in direct contact with the heme or the propionate groups, little effect on either intramolecular electron transfer or the heme potential | Homo sapiens |
Y83F | mutation is located on the surface of the heme domain, but not in direct contact with the heme or the propionate groups, little effect on either intramolecular electron transfer or the heme potential | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
sulfite | mutant Y83A, pH 8.0, 25°C | Homo sapiens | |
0.0028 | - |
sulfite | mutant H90F, pH 8.0, 25°C | Homo sapiens | |
0.0038 | - |
sulfite | mutant F79A, pH 8.0, 25°C | Homo sapiens | |
0.0039 | - |
sulfite | mutant H90Y, pH 8.0, 25°C | Homo sapiens | |
0.008 | - |
sulfite | mutant F57Y, pH 8.0, 25°C | Homo sapiens | |
0.011 | - |
sulfite | wild-type, pH 8.0, 25°C | Homo sapiens | |
0.012 | - |
sulfite | mutant Y83F, pH 8.0, 25°C | Homo sapiens | |
0.013 | - |
sulfite | mutant F57A, pH 8.0, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | sulfite | mutant Y83F, pH 8.0, 25°C | Homo sapiens | |
7 | - |
sulfite | mutant Y83A, pH 8.0, 25°C | Homo sapiens | |
13 | - |
sulfite | mutant F79A, pH 8.0, 25°C | Homo sapiens | |
16 | - |
sulfite | mutant F57A, pH 8.0, 25°C | Homo sapiens | |
19 | - |
sulfite | mutant F57Y, pH 8.0, 25°C | Homo sapiens | |
19 | - |
sulfite | mutant H90F, pH 8.0, 25°C | Homo sapiens | |
27 | - |
sulfite | wild-type, pH 8.0, 25°C | Homo sapiens | |
42 | - |
sulfite | mutant H90Y, pH 8.0, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | interactions of residue H90 with a heme propionate group destabilize the Fe(III) state of the heme | Homo sapiens |