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Literature summary for 1.8.3.1 extracted from

  • Davis, A.; Cornelison, M.; Meyers, K.; Rajapakshe, A.; Berry, R.; Tollin, G.; Enemark, J.
    Effects of mutating aromatic surface residues of the heme domain of human sulfite oxidase on its heme midpoint potential, intramolecular electron transfer, and steady-state kinetics (2013), Dalton Trans., 42, 3043-3049.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
F57A the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate Homo sapiens
F57Y the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate Homo sapiens
F79A the size and hydrophobicity of F57 play an important role in modulating the heme potential, residue F57 also affects the intramolecular electron transfer rate Homo sapiens
H90F interactions of H90 with a heme propionate group destabilize the Fe(III) state of the heme Homo sapiens
H90Y interactions of H90 with a heme propionate group destabilize the Fe(III) state of the heme Homo sapiens
Y83A mutation is located on the surface of the heme domain, but not in direct contact with the heme or the propionate groups, little effect on either intramolecular electron transfer or the heme potential Homo sapiens
Y83F mutation is located on the surface of the heme domain, but not in direct contact with the heme or the propionate groups, little effect on either intramolecular electron transfer or the heme potential Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
sulfite mutant Y83A, pH 8.0, 25°C Homo sapiens
0.0028
-
sulfite mutant H90F, pH 8.0, 25°C Homo sapiens
0.0038
-
sulfite mutant F79A, pH 8.0, 25°C Homo sapiens
0.0039
-
sulfite mutant H90Y, pH 8.0, 25°C Homo sapiens
0.008
-
sulfite mutant F57Y, pH 8.0, 25°C Homo sapiens
0.011
-
sulfite wild-type, pH 8.0, 25°C Homo sapiens
0.012
-
sulfite mutant Y83F, pH 8.0, 25°C Homo sapiens
0.013
-
sulfite mutant F57A, pH 8.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + cytochrome c
-
Homo sapiens sulfate + reduced cytochrome c
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 sulfite mutant Y83F, pH 8.0, 25°C Homo sapiens
7
-
sulfite mutant Y83A, pH 8.0, 25°C Homo sapiens
13
-
sulfite mutant F79A, pH 8.0, 25°C Homo sapiens
16
-
sulfite mutant F57A, pH 8.0, 25°C Homo sapiens
19
-
sulfite mutant F57Y, pH 8.0, 25°C Homo sapiens
19
-
sulfite mutant H90F, pH 8.0, 25°C Homo sapiens
27
-
sulfite wild-type, pH 8.0, 25°C Homo sapiens
42
-
sulfite mutant H90Y, pH 8.0, 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme interactions of residue H90 with a heme propionate group destabilize the Fe(III) state of the heme Homo sapiens