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Literature summary for 1.8.3.1 extracted from

  • Wilson, H.L.; Rajagopalan, K.V.
    The role of tyrosine 343 in substrate binding and catalysis by human sulfite oxidase (2004), J. Biol. Chem., 279, 15105-15113.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y343F in the mutant enzyme using cytochrome c as electron acceptor, turnover number is somewhat impaired, 34% of the wild-type activity at pH 8.5. The KM-value for the mutant enzyme shows a 5fold increase over wild-type. Reduction of the molybdenum center of the Y343 F variant by sulfite is more significantly impaired at high pH than at low pH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00129
-
sulfite 25°C, pH 6.0, 20 mM buffer, wild-type enzyme Homo sapiens
0.00162
-
sulfite 25°C, pH 6.5, 20 mM buffer, wild-type enzyme Homo sapiens
0.00272
-
sulfite 25°C, pH 7.0, 20 mM buffer, wild-type enzyme Homo sapiens
0.00311
-
sulfite 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00339
-
sulfite 25°C, pH 7.5, 20 mM buffer, wild-type enzyme Homo sapiens
0.00352
-
sulfite 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.00362
-
sulfite 25°C, pH 7.0, 100 mM buffer, wild-type enzyme Homo sapiens
0.00367
-
sulfite 25°C, pH 7.5, 100 mM buffer, wild-type enzyme Homo sapiens
0.00414
-
sulfite 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00423
-
sulfite 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.00435
-
sulfite 25°C, pH 8.0, 20 mM buffer, wild-type enzyme Homo sapiens
0.00459
-
sulfite 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00503
-
sulfite 25°C, pH 8.25, 20 mM buffer, wild-type enzyme Homo sapiens
0.00612
-
sulfite 25°C, pH 8.0, 100 mM buffer, wild-type enzyme Homo sapiens
0.00635
-
sulfite 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00728
-
sulfite 25°C, pH 8.25, 100 mM buffer, wild-type enzyme Homo sapiens
0.00825
-
sulfite 25°C, pH 8.5, 20 mM buffer, wild-type enzyme Homo sapiens
0.00851
-
sulfite 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00859
-
sulfite 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00908
-
sulfite 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00924
-
sulfite 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00947
-
sulfite 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00959
-
sulfite 25°C, pH 8.75, 20 mM buffer, wild-type enzyme Homo sapiens
0.00963
-
sulfite 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.00992
-
sulfite 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F Homo sapiens
0.011
-
sulfite 25°C, pH 8.5, 100 mM buffer, wild-type enzyme Homo sapiens
0.0158
-
sulfite 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.0221
-
sulfite 25°C, pH 9.0, 20 mM buffer, wild-type enzyme Homo sapiens
0.026
-
sulfite 25°C, pH 9.0, 100 mM buffer, wild-type enzyme Homo sapiens
0.0319
-
sulfite 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.0536
-
sulfite 25°C, pH 9.5, 100 mM buffer, wild-type enzyme Homo sapiens
0.0557
-
sulfite 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.0671
-
sulfite 25°C, pH 9.5, 20 mM buffer, wild-type enzyme Homo sapiens
0.147
-
sulfite 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
0.59 1 sulfite 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.54
-
sulfite 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + O2 + H2O
-
Homo sapiens sulfate + H2O2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.06
-
sulfite 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
3.11
-
sulfite 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
3.26
-
sulfite 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
4.14
-
sulfite 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
4.59
-
sulfite 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
6.35
-
sulfite 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
7.17
-
sulfite 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
8.21
-
sulfite 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
8.51
-
sulfite 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
8.59
-
sulfite 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
8.72
-
sulfite 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F Homo sapiens
9.08
-
sulfite 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F Homo sapiens
9.2
-
sulfite 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
9.24
-
sulfite 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
9.47
-
sulfite 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
9.63
-
sulfite 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
9.92
-
sulfite 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F Homo sapiens
9.99
-
sulfite 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
10.5
-
sulfite 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
12.1
-
sulfite 25°C, pH 7.0, 100 mM buffer, wild-type enzyme Homo sapiens
13.2
-
sulfite 25°C, pH 6.0, 20 mM buffer, wild-type enzyme Homo sapiens
17.2
-
sulfite 25°C, pH 7.5, 100 mM buffer, wild-type enzyme Homo sapiens
17.7
-
sulfite 25°C, pH 6.5, 20 mM buffer, wild-type enzyme Homo sapiens
23.6
-
sulfite 25°C, pH 8.75, 20 mM buffer, wild-type enzyme Homo sapiens
24.2
-
sulfite 25°C, pH 7.0, 20 mM buffer, wild-type enzyme Homo sapiens
24.6
-
sulfite 25°C, pH 9.5, 100 mM buffer, wild-type enzyme Homo sapiens
24.7
-
sulfite 25°C, pH 7.5, 20 mM buffer, wild-type enzyme Homo sapiens
24.8
-
sulfite 25°C, pH 8.25, 20 mM buffer, wild-type enzyme Homo sapiens
25
-
sulfite 25°C, pH 8.0, 100 mM buffer, wild-type enzyme Homo sapiens
25.7
-
sulfite 25°C, pH 9.0, 20 mM buffer, wild-type enzyme Homo sapiens
25.9
-
sulfite 25°C, pH 8.0, 20 mM buffer, wild-type enzyme Homo sapiens
26.3
-
sulfite 25°C, pH 9.5, 20 mM buffer, wild-type enzyme Homo sapiens
26.9
-
sulfite 25°C, pH 8.5, 100 mM buffer, wild-type enzyme Homo sapiens
26.9
-
sulfite 25°C, pH 8.5, 20 mM buffer, wild-type enzyme Homo sapiens
27
-
sulfite 25°C, pH 8.25, 100 mM buffer, wild-type enzyme Homo sapiens
28.1
-
sulfite 25°C, pH 9.0, 100 mM buffer, wild-type enzyme Homo sapiens