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Literature summary for 1.8.2.4 extracted from
- Dimethylsulfide: acceptor oxidoreductase from Rhodobacter sulfidophilus - The purified enzyme contains b-type haem and a pterin molybdenum cofactor (1996), Eur. J. Biochem., 239, 391-396.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Rhodovulum sulfidophilum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | the enzyme contains 3.5 mol Fe per mol enzyme | Rhodovulum sulfidophilum |  |
| Molybdenum | the enzyme contains 0.5 mol molybdenum per mol enzyme | Rhodovulum sulfidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
| 38000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
| 94000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
| 152000 | - |
gel filtration | Rhodovulum sulfidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodovulum sulfidophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration | Rhodovulum sulfidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.2 | - |
crude extract, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication | Rhodovulum sulfidophilum |
| 20.2 | - |
after purification, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication | Rhodovulum sulfidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethyl sulfoxide + reduced 2,6-dichloroindophenol | - |
Rhodovulum sulfidophilum | dimethyl sulfide + oxidized 2,6-dichloroindophenol + H2O | - |
? | |
| dimethyl sulfoxide + reduced methyl viologen + H2O | 2% activity compared to trimethylamine N-oxide | Rhodovulum sulfidophilum | dimethyl sulfide + oxidized methyl viologen | - |
? | |
| lauryldimethylamine N-oxide + reduced methyl viologen + H2O | 69% activity compared to trimethylamine N-oxide | Rhodovulum sulfidophilum | ? | - |
? | |
| additional information | no activity with chlorate and bromate | Rhodovulum sulfidophilum | ? | - |
? | |
| trimethylamine N-oxide + reduced methyl viologen + H2O | 100% activity | Rhodovulum sulfidophilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | 1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dimethylsulfide: acceptor oxidoreductase | - |
Rhodovulum sulfidophilum |
| Me2S:acceptor oxidoreductase | - |
Rhodovulum sulfidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | the cytochrome contains a b-type heme and a content of 0.65 mol protoheme per mol enzyme is determined | Rhodovulum sulfidophilum | |
| iron-sulfur centre | - |
Rhodovulum sulfidophilum | |
| molybdopterin | - |
Rhodovulum sulfidophilum | |
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Release 2023.1 (January 2023)
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