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Literature summary for 1.8.2.2 extracted from

  • Kurek, E.J.
    Properties of an enzymatic complex active in sulfite and thiosulfate oxidation by Rhodotorula sp. (1985), Arch. Microbiol., 143, 277-282.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 17% inhibition at 5 mM Rhodotorula sp.
2,2'-bipyridyl not inhibitory at 5 mM Rhodotorula sp.
deoxycholate
-
Rhodotorula sp.
HgCl2 100% inhibition at 0.5 mM, 45% inhibition at 0.25 mM and 15% inhibition at 0.1 mM Rhodotorula sp.
N-ethylmaleimide 12% inhibition at 1 mM Rhodotorula sp.
p-hydroxymercuribenzoate 50% inhibition at 1 mM, 40% inhibition at 0.5 mM and 10% inhibition at 0.1 mM Rhodotorula sp.
SO32-
-
Rhodotorula sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
thiosulfate with native cytochrome c as electron acceptor Rhodotorula sp.
0.16
-
thiosulfate with ferricyanide as electron acceptor Rhodotorula sp.
0.34
-
ferricyanide
-
Rhodotorula sp.

Organism

Organism UniProt Comment Textmining
Rhodotorula sp.
-
-
-

Storage Stability

Storage Stability Organism
-20°C, the activity of cell-free extracts is stable for several days in the presence of 20% v/v glycerol, without glycerol most of the activity is lost after 2 days Rhodotorula sp.
-20°C, the activity of whole cells is stable for 3 months Rhodotorula sp.
4°C, cell-free extracts lose most of the activity after 2 days even in the presence of glycerol Rhodotorula sp.
the activity in cell-free extracts is lost very rapidly after freezing and thawing Rhodotorula sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + ferricyanide
-
Rhodotorula sp. tetrathionate + ferrocyanide
-
?
thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Rhodotorula sp. tetrathionate + reduced acceptor
-
?
thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?
thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?
thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Rhodotorula sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30% decrease of activity after 5 min Rhodotorula sp.
60
-
50% decrease of activity after 7 min Rhodotorula sp.
100
-
after 2 min the activity is completely destroyed Rhodotorula sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Rhodotorula sp.