Crystallization (Comment) | Organism |
---|---|
crystal structure | Starkeya novella |
Protein Variants | Comment | Organism |
---|---|---|
H57A | mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate | Starkeya novella |
R55M | mutant with reduced activity, R-55 is an important position close to the substrate binding site, where it makes hydrogen bonds to the equatorial oxo ligand of the molybdenum, to Gln-33, and a nearby water molecule. It also forms a salt bridge, comprising two hydrogen bonds, with propionate-6 of the heme moiety of the cytochrome subunit | Starkeya novella |
Y236F | mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate | Starkeya novella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F | Starkeya novella | |
0.000007 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F | Starkeya novella | |
0.0006 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme | Starkeya novella | |
0.0011 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme | Starkeya novella | |
0.0037 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme | Starkeya novella | |
0.0071 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme | Starkeya novella | |
0.022 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme | Starkeya novella | |
0.026 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F | Starkeya novella | |
0.042 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F | Starkeya novella | |
0.086 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme | Starkeya novella | |
0.114 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F | Starkeya novella | |
0.189 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A | Starkeya novella | |
0.22 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A | Starkeya novella | |
0.27 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A | Starkeya novella | |
0.29 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A | Starkeya novella | |
0.324 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme | Starkeya novella | |
0.332 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236Fyme | Starkeya novella | |
0.452 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A | Starkeya novella | |
0.667 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A | Starkeya novella | |
0.812 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M | Starkeya novella | |
1.087 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M | Starkeya novella | |
1.155 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F | Starkeya novella | |
1.46 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A | Starkeya novella | |
1.63 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M | Starkeya novella | |
1.66 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme | Starkeya novella | |
1.95 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M | Starkeya novella | |
3.39 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme | Starkeya novella | |
3.62 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M | Starkeya novella | |
4.456 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F | Starkeya novella | |
8.17 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M | Starkeya novella | |
12.2 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A | Starkeya novella | |
15.49 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F | Starkeya novella |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Starkeya novella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | Starkeya novella | - |
sulfate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | Q9LA16 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | - |
Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | cytochrome c from horse heart | Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Starkeya novella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme | Starkeya novella | |
36.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F | Starkeya novella | |
41.6 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F | Starkeya novella | |
48.3 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F | Starkeya novella | |
51.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F | Starkeya novella | |
52.7 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F | Starkeya novella | |
53.4 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F | Starkeya novella | |
58.3 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F | Starkeya novella | |
63.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme | Starkeya novella | |
64 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M | Starkeya novella | |
64 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M | Starkeya novella | |
64 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F | Starkeya novella | |
66.3 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M | Starkeya novella | |
68.3 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M | Starkeya novella | |
70.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M | Starkeya novella | |
73.4 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M | Starkeya novella | |
86.2 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme | Starkeya novella | |
88.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F | Starkeya novella | |
148.4 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A | Starkeya novella | |
158.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme | Starkeya novella | |
192.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A | Starkeya novella | |
214.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A | Starkeya novella | |
214.6 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A | Starkeya novella | |
220.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A | Starkeya novella | |
222.4 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A | Starkeya novella | |
226.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A | Starkeya novella | |
238.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A | Starkeya novella | |
293.4 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme | Starkeya novella | |
345.3 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme | Starkeya novella | |
410 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme | Starkeya novella | |
431 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme | Starkeya novella | |
519 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme | Starkeya novella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Starkeya novella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | less than 50% of maximal activity above and below | Starkeya novella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | - |
Starkeya novella | |
molybdenum cofactor | (molybdopterin-S,S)-oxo-molybdenum | Starkeya novella |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.84 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F | Starkeya novella | |
8.98 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M | Starkeya novella | |
11.6 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F | Starkeya novella | |
18.9 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M | Starkeya novella | |
34 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M | Starkeya novella | |
54.5 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F | Starkeya novella | |
58.9 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M | Starkeya novella | |
67.7 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme | Starkeya novella | |
86.8 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M | Starkeya novella | |
122 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A | Starkeya novella | |
132 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A | Starkeya novella | |
173 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F | Starkeya novella | |
251 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme | Starkeya novella | |
358 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A | Starkeya novella | |
461 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F | Starkeya novella | |
492 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A | Starkeya novella | |
782 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A | Starkeya novella | |
799 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A | Starkeya novella | |
1010 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A | Starkeya novella | |
1130 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A | Starkeya novella | |
1140 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F | Starkeya novella | |
1500 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme | Starkeya novella | |
1970 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F | Starkeya novella | |
3930 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M | Starkeya novella | |
4880 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme | Starkeya novella | |
5600 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F | Starkeya novella | |
8420 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F | Starkeya novella | |
15300 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme | Starkeya novella | |
40300 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme | Starkeya novella | |
42700 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme | Starkeya novella | |
75500 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme | Starkeya novella | |
106000 | - |
sulfite | 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme | Starkeya novella |