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Literature summary for 1.8.2.1 extracted from
- Bacterial sulfite dehydrogenases in organotrophic metabolism: separation and identification in Cupriavidus necator H16 and in Delftia acidovorans SPH-1 (2008), Microbiology, 154, 256-263.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | N-terminal amino acid sequence of the mature SorA from Cupriavidus necator H16 is currently unique | Cupriavidus necator H16 |
| additional information | the N-terminal amino acid sequence of the mature SorA from Delftia acidovorans SPH-1 is found in about 500 proteins | Delftia acidovorans SPH-1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | SorA | Cupriavidus necator H16 | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39800 | - |
sequence analysis | Delftia acidovorans SPH-1 |
| 40000 | - |
denatured protein, sequence analysis | Cupriavidus necator H16 |
| 40000 | - |
2 * 40000, SDS-PAGE | Delftia acidovorans SPH-1 |
| 40700 | - |
sequence analysis | Cupriavidus necator H16 |
| 67000 | - |
gel filtration, SorA | Delftia acidovorans SPH-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator H16 | - |
- |
- |
| Delftia acidovorans SPH-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by anion-exchange chromatography and gel filtration, 270fold | Delftia acidovorans SPH-1 |
| by anion-exchange chromatography and gel filtration, 320fold | Cupriavidus necator H16 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, MES, phosphate, Tris or CAPS buffer, pH 5.5-10.5, for at least 3 months | Cupriavidus necator H16 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + ferricyanide + H2O | - |
Cupriavidus necator H16 | sulfate + ferrocyanide + H+ | - |
? |  |
| sulfite + ferricyanide + H2O | - |
Delftia acidovorans SPH-1 | sulfate + ferrocyanide + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 40000, SDS-PAGE | Delftia acidovorans SPH-1 |
| monomer | - |
Cupriavidus necator H16 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SorA | - |
Cupriavidus necator H16 |
| SorA | - |
Delftia acidovorans SPH-1 |
| sulfite dehydrogenase | - |
Cupriavidus necator H16 |
| sulfite dehydrogenase | - |
Delftia acidovorans SPH-1 |
| sulfite oxidoreductase | - |
Delftia acidovorans SPH-1 |
| sulfite: ferricytochrome-c oxidoreductase | - |
Cupriavidus necator H16 |
| sulfite: ferricytochrome-c oxidoreductase | - |
Delftia acidovorans SPH-1 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Delftia acidovorans SPH-1 | - |
- |
8.7 |
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