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Literature summary for 1.8.2.1 extracted from

  • Kappler, U.; Bailey, S.; Feng, C.; Honeychurch, M.J.; Hanson, G.R.; Bernhardt, P.V.; Tollin, G.; Enemark, J.H.
    Kinetic and structural evidence for the importance of Tyr236 for the integrity of the Mo active site in a bacterial sulfite dehydrogenase (2006), Biochemistry, 45, 9696-9705.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DH5alpha Starkeya novella

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Starkeya novella

Protein Variants

Protein Variants Comment Organism
Y236F reduced turnover rates and substrate affinity as well as an altered reactivity toward molecular oxygen as an electron acceptor, unlike the wild type enzyme the mutant enzyme is reoxidized quickly in the presence of molecular oxygen Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Starkeya novella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
wild type enzyme, analytical ultracentrifugation Starkeya novella
43000
-
mutant enzyme Y236F, analytical ultracentrifugation Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE anion exchange column chromatography Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricytochrome c + H2O
-
Starkeya novella sulfate + ferrocytochrome c + H+
-
?

Subunits

Subunits Comment Organism
heterodimer
-
Starkeya novella

Synonyms

Synonyms Comment Organism
SDH
-
Starkeya novella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
sulfite mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella
0.8
-
sulfite mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
33.51
-
sulfite wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
36.19
-
sulfite mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
52.51
-
sulfite mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella
333.7
-
sulfite wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.4 wild type enzyme Starkeya novella
7.2 8.64 mutant enzyme Y236F Starkeya novella

Cofactor

Cofactor Comment Organism Structure
heme c
-
Starkeya novella