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Literature summary for 1.8.1.B5 extracted from
- Structural and biochemical characterizations of methanoredoxin from Methanosarcina acetivorans, a glutaredoxin-like enzyme with coenzyme M-dependent protein disulfide reductase activity (2016), Biochemistry, 55, 313-321.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. A pocket on the surface contains a CVWC motif, identifying the active site with architecture similar to glutaredoxins. Active site modeling of coenzyme M shows the sulfate moiety hydrogen-bonded to the backbone amide and carbonyl oxygen of residue Phe 76 | Methanosarcina acetivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
reduced coenzyme M | pH 6.8, temperature not specified in the publication | Methanosarcina acetivorans |  |
| 8.9 | - |
reduced glutathione | pH 6.8, temperature not specified in the publication | Methanosarcina acetivorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | a cadmium ion is found within the active site of each monomer, crystallization data | Methanosarcina acetivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11500 | - |
1 * 11500, SDS-PAGE | Methanosarcina acetivorans |
| 12000 | - |
gel filtration | Methanosarcina acetivorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina acetivorans | Q8TQ93 | - |
- |
| Methanosarcina acetivorans DSM 2834 | Q8TQ93 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin disulfide + reduced coenzyme M | - |
Methanosarcina acetivorans | insulin dithiol + oxidized coenzyme M | - |
? | |
| insulin disulfide + reduced coenzyme M | - |
Methanosarcina acetivorans DSM 2834 | insulin dithiol + oxidized coenzyme M | - |
? | |
| insulin disulfide + reduced glutathione | - |
Methanosarcina acetivorans | insulin dithiol + oxidized glutathione | - |
? | |
| insulin disulfide + reduced glutathione | - |
Methanosarcina acetivorans DSM 2834 | insulin dithiol + oxidized glutathione | - |
? | |
| additional information | no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity | Methanosarcina acetivorans | ? | - |
? | |
| additional information | no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity | Methanosarcina acetivorans DSM 2834 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 11500, SDS-PAGE | Methanosarcina acetivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MA_1658 | - |
Methanosarcina acetivorans |
| methanoredoxin | - |
Methanosarcina acetivorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme M | coenzyme M-SH and glutathione bind to the active site | Methanosarcina acetivorans | |
| glutathione | coenzyme M-SH and glutathione bind to the active site | Methanosarcina acetivorans | |
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