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Literature summary for 1.8.1.9 extracted from

  • McCarver, A.C.; Lessner, D.J.
    Molecular characterization of the thioredoxin system from Methanosarcina acetivorans (2014), FEBS J., 281, 4598-7611.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina acetivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
0.086
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
0.736
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thioredoxin disulfide + NADPH + H+ Methanosarcina acetivorans
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 activity with 5,5'-dithiobis(2-nitrobenzoic acid) and NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
0.5
-
 activity with 5,5'-dithiobis(2-nitrobenzoic acid) and NADH, pH and temperature not specified in the publication Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Methanosarcina acetivorans 2-nitro-5-thiobenzoate + NAD+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Methanosarcina acetivorans 2-nitro-5-thiobenzoate + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Methanosarcina acetivorans thioredoxin + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ the enzyme is specific for Methanosarcina acetivorans thioredoxin 7, no activity with Methanosarcina acetivorans thioredoxin 2 or Methanosarcina acetivorans thioredoxin 6 Methanosarcina acetivorans thioredoxin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
thioredoxin reductase
-
 Methanosarcina acetivorans
TrxR
-
 Methanosarcina acetivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
0.817
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
1.175
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Methanosarcina acetivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
13.7
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
103.3
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans